Literature DB >> 6331315

Ghost cells as sorption matrix for virus concentration from water.

R Armon, Y Kott, I Neeman.   

Abstract

Pig erythrocyte membranes were used as adsorbent material for the concentration of bacteriophage phi x-174, MS-2, and f2 from 5 ml of saline solution. The adsorption was carried out at pH 3.5, and the elution was carried out at pH 7.0. Compared with adsorption on 3% beef extract, bacteriophage adsorption on erythrocyte ghost cells yielded 93 to 100%, and elution was 92 to 100%, whereas beef extract organic flocculation yielded adsorption of 0 to 98.8% and elution of 61 to 86.6%. The same procedure but with poliovirus LSC-1 gave 100% adsorption and 91 to 129% elution.

Entities:  

Mesh：

Year:  1984        PMID： 6331315      PMCID： PMC240238          DOI： 10.1128/aem.47.6.1337-1340.1984

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  10 in total

1.  Organic flocculation: an efficient second-step concentration method for the detection of viruses in tap water.

Authors:  E Katzenelson; B Fattal; T Hostovesky
Journal:  Appl Environ Microbiol       Date:  1976-10       Impact factor: 4.792

2.  The replication of bacteriophage MS2. 1. Transfer of parental nucleic acid to progeny phage.

Authors:  J E DAVIS; R L SINSHEIMER
Journal:  J Mol Biol       Date:  1963-03       Impact factor: 5.469

3.  The contribution of sialic acid to the surface charge of the erythrocyte.

Authors:  E H EYLAR; M A MADOFF; O V BRODY; J L ONCLEY
Journal:  J Biol Chem       Date:  1962-06       Impact factor: 5.157

4.  A bacteriophage containing RNA.

Authors:  T LOEB; N D ZINDER
Journal:  Proc Natl Acad Sci U S A       Date:  1961-03-15       Impact factor: 11.205

5.  Adsorption studies of enzymes and other proteins.

Authors:  C A ZITTLE
Journal:  Adv Enzymol Relat Subj Biochem       Date:  1953

6.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

7.  Concentration of bacteriophages from natural waters.

Authors:  N D Seeley; S B Primrose
Journal:  J Appl Bacteriol       Date:  1979-02

8.  Isolation of human erythrocyte membranes and their outer surface proteins by covalent fractionation on solid support.

Authors:  Y Eshdat; A Prujansky-Jakobovits
Journal:  FEBS Lett       Date:  1979-05-01       Impact factor: 4.124

9.  Concentration of coliphages from large volumes of water and wastewater.

Authors:  S M Goyal; K S Zerda; C P Gerba
Journal:  Appl Environ Microbiol       Date:  1980-01       Impact factor: 4.792

Review 10.  Virus-erythrocyte interactions.

Authors:  C Howe; L T Lee
Journal:  Adv Virus Res       Date:  1972       Impact factor: 9.937
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.