Literature DB >> 63310

Differential fluorescence of sister chromatids with 4'-6-diamidino-2-phenylindole.

M S Lin, O S Alfi, G N Donnell.   

Abstract

Differential fluorescence of sister chromatids and sister chromatid exchanges (SCE) in chromosomes from human lymphocytes grown two replication cycles in medium containing 5-bromodeoxyuridine can be detected by fluorescence microscopy after staining with 4'-6-diamidino-2-phenylindole (DAPI). The DAPI fluorescence appears to be more stable than that of the dye 33258 Hoechst and may provide a more sensitive method for the detection of SCE.

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Year:  1976        PMID： 63310     DOI： 10.1139/g76-068

Source DB:  PubMed          Journal:  Can J Genet Cytol        ISSN： 0008-4093


  6 in total

1.  Interactions of species in an Anabaena flos-aquae association from the Plußsee (East-Holstein, Federal Republic of Germany).

Authors:  Ute Müller; Peter V Sengbusch
Journal:  Oecologia       Date:  1983-05       Impact factor: 3.225

2.  Different effects of 33258 Hoechst and DAPI in fluorescent staining of sister chromatids differentially substituted with bromodeoxyuridine.

Authors:  C H Buys; A Y van der Veen
Journal:  Histochemistry       Date:  1982

3.  Involvement of sulfhydryl groups of chromosomal proteins in sister chromatid differentiation.

Authors:  C H Buys; S Stienstra
Journal:  Chromosoma       Date:  1980       Impact factor: 4.316

4.  Interactions of 4', 6-diamidine-2-phenylindole with synthetic polynucleotides.

Authors:  J Kapuściński; W Szer
Journal:  Nucleic Acids Res       Date:  1979-08-10       Impact factor: 16.971

5.  The role of chromosomal proteins in the induction of a differential staining of sister chromatids by light.

Authors:  C H Buys; J Osinga
Journal:  Histochem J       Date:  1981-09

6.  Optical Studies of the interaction of 4'-6'-diamidino-2-phenylindole with DNA and metaphase chromosomes.

Authors:  M S Lin; D E Comings; O S Alfi
Journal:  Chromosoma       Date:  1977-03-07       Impact factor: 4.316
  6 in total

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