Literature DB >> 6330533

Enhanced transformation of human cells by UV-irradiated pSV2 plasmids.

G Spivak, A K Ganesan, P C Hanawalt.   

Abstract

Irradiating the plasmid pSV2-gpt with UV (254 nm) doses up to 200 J m-2 caused a dose-dependent increase in the yield of Gpt+ transformants when the plasmid was introduced into human cells by calcium phosphate coprecipitation. UV doses greater than 1 kJ m-2 were required to reduce the efficiency of transformation below that obtained with unirradiated DNA.

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Year:  1984        PMID： 6330533      PMCID： PMC368887          DOI： 10.1128/mcb.4.6.1169-1171.1984

Source DB:  PubMed          Journal:  Mol Cell Biol        ISSN： 0270-7306            Impact factor:   4.272


  22 in total

1.  Antagonists of DNA gyrase inhibit repair and recombination of UV-irradiated phage lambda.

Authors:  J B Hays; S Boehmer
Journal:  Proc Natl Acad Sci U S A       Date:  1978-09       Impact factor: 11.205

2.  Genetic exchanges caused by ultraviolet photoproducts in phage lambda DNA molecules: the role of DNA replication.

Authors:  P F Lin; P Howard-Flanders
Journal:  Mol Gen Genet       Date:  1976-07-23

3.  Improved microfluorometric DNA determination in biological material using 33258 Hoechst.

Authors:  C F Cesarone; C Bolognesi; L Santi
Journal:  Anal Biochem       Date:  1979-11-15       Impact factor: 3.365

4.  Recombination between temperature-sensitive mutants of simian virus 40.

Authors:  D R Dubbs; M Rachmeler; S Kit
Journal:  Virology       Date:  1974-01       Impact factor: 3.616

5.  Inhibition of SV40 replication in simian cells by specific pBR322 DNA sequences.

Authors:  M Lusky; M Botchan
Journal:  Nature       Date:  1981-09-03       Impact factor: 49.962

6.  Binding of T4 endonuclease V to deoxyribonucleic acid irradiated with ultraviolet light.

Authors:  P C Seawell; T J Simon; A K Ganesan
Journal:  Biochemistry       Date:  1980-04-15       Impact factor: 3.162

7.  Regulation of simian virus 40 transcription: sensitive analysis of the RNA species present early in infections by virus or viral DNA.

Authors:  B A Parker; G R Stark
Journal:  J Virol       Date:  1979-08       Impact factor: 5.103

8.  Characteristics of an SV40-plasmid recombinant and its movement into and out of the genome of a murine cell.

Authors:  D Hanahan; D Lane; L Lipsich; M Wigler; M Botchan
Journal:  Cell       Date:  1980-08       Impact factor: 41.582

9.  Expression of a bacterial gene in mammalian cells.

Authors:  R C Mulligan; P Berg
Journal:  Science       Date:  1980-09-19       Impact factor: 47.728

10.  Isolation of supercoiled colicinogenic factor E 1 DNA sensitive to ribonuclease and alkali.

Authors:  D G Blair; D J Sherratt; D B Clewell; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1972-09       Impact factor: 11.205
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  15 in total

1.  Abnormal response to DNA crosslinking agents of Fanconi anemia fibroblasts can be corrected by transfection with normal human DNA.

Authors:  C Diatloff-Zito; D Papadopoulo; D Averbeck; E Moustacchi
Journal:  Proc Natl Acad Sci U S A       Date:  1986-09       Impact factor: 11.205

2.  Enhancement of transformation rates in higher plants by low-dose irradiation: Are DNA repair systems involved in the incorporation of exogenous DNA into the plant genome?

Authors:  F Köhler; G Cardon; M Pöhlman; R Gill; O Schieder
Journal:  Plant Mol Biol       Date:  1989-02       Impact factor: 4.076

3.  Recombination between irradiated shuttle vector DNA and chromosomal DNA in African green monkey kidney cells.

Authors:  J S Mudgett; W D Taylor
Journal:  Mol Cell Biol       Date:  1990-01       Impact factor: 4.272

4.  Hybrid genes in the analysis of transformation conditions. 3. Temporal/spatial fate of NPTII gene integration, its inheritance and factors affecting these processes in Nicotiana plumbaginifolia.

Authors:  G B Gharti-Chhetri; W Cherdshewasart; J Dewulf; J Paszkowski; M Jacobs; I Negrutiu
Journal:  Plant Mol Biol       Date:  1990-05       Impact factor: 4.076

5.  A comparison of calcium phosphate coprecipitation and electroporation. Implications for studies on the genetic effects of DNA damage.

Authors:  J A Nickoloff; L N Spirio; R J Reynolds
Journal:  Mol Biotechnol       Date:  1998-10       Impact factor: 2.695

6.  Frequency of intrachromosomal homologous recombination induced by UV radiation in normally repairing and excision repair-deficient human cells.

Authors:  T Tsujimura; V M Maher; A R Godwin; R M Liskay; J J McCormick
Journal:  Proc Natl Acad Sci U S A       Date:  1990-02       Impact factor: 11.205

7.  Transfer of human genes conferring resistance to methylating mutagens, but not to UV irradiation and cross-linking agents, into Chinese hamster ovary cells.

Authors:  B Kaina; A A Van Zeeland; C Backendorf; H W Thielmann; P Van de Putte
Journal:  Mol Cell Biol       Date:  1987-05       Impact factor: 4.272

8.  UV stimulation of DNA-mediated transformation of human cells.

Authors:  M van Duin; A Westerveld; J H Hoeijmakers
Journal:  Mol Cell Biol       Date:  1985-04       Impact factor: 4.272

9.  Ep-CAM: a human epithelial antigen is a homophilic cell-cell adhesion molecule.

Authors:  S V Litvinov; M P Velders; H A Bakker; G J Fleuren; S O Warnaar
Journal:  J Cell Biol       Date:  1994-04       Impact factor: 10.539

10.  MMTV/LTR Promoter-Driven Transgenic Expression of EpCAM Leads to the Development of Large Pancreatic Islets.

Authors:  Jeffrey R Vercollone; Maarten Balzar; Sergey V Litvinov; Wendy Yang; Vincenzo Cirulli
Journal:  J Histochem Cytochem       Date:  2015-08       Impact factor: 2.479
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