Literature DB >> 6330369

In vitro replication of a dam methylated and non-methylated ori-C plasmid.

P Hughes, F Squali-Houssaini, P Forterre, M Kohiyama.   

Abstract

We have examined the replication of a dam methylated and non-methylated ori-C plasmid in an in vitro ori-C dependent replication system. The results show that the non-methylated plasmid is 50% to 80% less efficient in the initiation of DNA synthesis; that the methylation state of the plasmid does not change the site of initiation at ori-C, and that in both cases initiation at this region requires the presence of exogenously furnished dnaA protein.

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Year:  1984        PMID： 6330369     DOI： 10.1016/0022-2836(84)90386-3

Source DB:  PubMed          Journal:  J Mol Biol        ISSN： 0022-2836            Impact factor:   5.469


  17 in total

1.  The hemimethylated replication origin of Escherichia coli can be initiated in vitro.

Authors:  E Boye
Journal:  J Bacteriol       Date:  1991-07       Impact factor: 3.490

2.  Inhibition of DNA synthesis at the hemimethylated pBR322 origin of replication by a cell membrane fraction.

Authors:  A Malki; R Kern; M Kohiyama; P Hughes
Journal:  Nucleic Acids Res       Date:  1992-01-11       Impact factor: 16.971

3.  The FIS protein binds and bends the origin of chromosomal DNA replication, oriC, of Escherichia coli.

Authors:  H Gille; J B Egan; A Roth; W Messer
Journal:  Nucleic Acids Res       Date:  1991-08-11       Impact factor: 16.971

4.  dam methylation and the initiation of DNA replication on oriC plasmids.

Authors:  A Landoulsi; P Hughes; R Kern; M Kohiyama
Journal:  Mol Gen Genet       Date:  1989-04

5.  DNA methylation differentially enhances the expression of one of the two E. coli dnaA promoters in vivo and in vitro.

Authors:  R E Braun; A Wright
Journal:  Mol Gen Genet       Date:  1986-02

6.  Regulation of transcription of the chromosomal dnaA gene of Escherichia coli.

Authors:  C Kücherer; H Lother; R Kölling; M A Schauzu; W Messer
Journal:  Mol Gen Genet       Date:  1986-10

7.  Biological role of DNA methylation: sequence-specific single-strand breaks associated with hypomethylation of GATC sites in Escherichia coli DNA.

Authors:  M Szyf; E Meisels; A Razin
Journal:  J Bacteriol       Date:  1986-12       Impact factor: 3.490

8.  Evidence of two levels of control of P1 oriR and host oriC replication origins by DNA adenine methylation.

Authors:  A Abeles; T Brendler; S Austin
Journal:  J Bacteriol       Date:  1993-12       Impact factor: 3.490

9.  Dam methylated and hemimethylated oriC plasmids are replicated symmetrically; a novel and general test of replication symmetry.

Authors:  P Hughes; A Landoulsi; R Kern; M Kohiyama
Journal:  Mol Gen Genet       Date:  1989-06

10.  The distribution and properties of RNA primed initiation sites of DNA synthesis at the replication origin of Escherichia coli chromosome.

Authors:  Y Kohara; N Tohdoh; X W Jiang; T Okazaki
Journal:  Nucleic Acids Res       Date:  1985-10-11       Impact factor: 16.971
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