Literature DB >> 6330146

Viradel method for detection of rotavirus from seawater.

S M Goyal, C P Gerba.   

Abstract

This report describes the use of a virus adsorption-elution (Viradel) technique for the concentration and detection of rotavirus from seawater. Simian rotavirus SA-11 was used as a model virus because it can be readily propagated and plaqued in vitro. The virus adsorbed optimally to fiberglass epoxy filters when seawater was adjusted to pH 3.5 and aluminum chloride added to a concentration of 1.0 mM. Adsorbed virus was eluted with 6% beef extract at pH 10.5 and was further concentrated to a smaller volume by a modification of the organic flocculation method. Using this method, in conjunction with an indirect immunofluorescence test, rotavirus was detected in sewage-contaminated seawater.

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Year:  1983        PMID： 6330146     DOI： 10.1016/0166-0934(83)90080-0

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  11 in total

1.  Molecular assays for targeting human and bovine enteric viruses in coastal waters and their application for library-independent source tracking.

Authors:  Theng-Theng Fong; Dale W Griffin; Erin K Lipp
Journal:  Appl Environ Microbiol       Date:  2005-04       Impact factor: 4.792

2.  Concentration of enteroviruses, adenoviruses, and noroviruses from drinking water by use of glass wool filters.

Authors:  Elisabetta Lambertini; Susan K Spencer; Phillip D Bertz; Frank J Loge; Burney A Kieke; Mark A Borchardt
Journal:  Appl Environ Microbiol       Date:  2008-03-21       Impact factor: 4.792

3.  Simple method for the detoxification of wastewater ultrafiltration concentrates for rotavirus assay by indirect immunofluorescence.

Authors:  J I Oragui; D D Mara
Journal:  Appl Environ Microbiol       Date:  1989-02       Impact factor: 4.792

4.  Comparison of genomic homologies in the coxsackievirus B group by use of cDNA:RNA dot-blot hybridization.

Authors:  S Tracy
Journal:  J Clin Microbiol       Date:  1985-03       Impact factor: 5.948

5.  Development of a method for concentration of rotavirus and its application to recovery of rotaviruses from estuarine waters.

Authors:  V C Rao; T G Metcalf; J L Melnick
Journal:  Appl Environ Microbiol       Date:  1986-09       Impact factor: 4.792

6.  Human pathogenic viruses at sewage sludge disposal sites in the Middle Atlantic region.

Authors:  S M Goyal; W N Adams; M L O'Malley; D W Lear
Journal:  Appl Environ Microbiol       Date:  1984-10       Impact factor: 4.792

Review 7.  Pathogenic human viruses in coastal waters.

Authors:  Dale W Griffin; Kim A Donaldson; John H Paul; Joan B Rose
Journal:  Clin Microbiol Rev       Date:  2003-01       Impact factor: 26.132

8.  No influence of oxygen levels on pathogenesis and virus shedding in Salmonid alphavirus (SAV)-challenged Atlantic salmon (Salmo salar L.).

Authors:  Linda Andersen; Kjartan Hodneland; Are Nylund
Journal:  Virol J       Date:  2010-08-21       Impact factor: 4.099

9.  Prevalence of Rotavirus Genogroup A and Norovirus Genogroup II in Bassaseachic Falls National Park Surface Waters in Chihuahua, Mexico.

Authors:  Ma Carmen E Delgado-Gardea; Patricia Tamez-Guerra; Ricardo Gomez-Flores; Aurora Mendieta-Mendoza; Francisco Javier Zavala-Díaz de la Serna; Juan Francisco Contreras-Cordero; Gilberto Erosa-de la Vega; María Concepción Pérez-Recoder; Blanca Sánchez-Ramírez; Carmen González-Horta; Rocío Infante-Ramírez
Journal:  Int J Environ Res Public Health       Date:  2017-05-05       Impact factor: 3.390

10.  Atlantic salmon (Salmo salar L.) post-smolts challenged two or nine weeks after seawater-transfer show differences in their susceptibility to salmonid alphavirus subtype 3 (SAV3).

Authors:  J Jarungsriapisit; L J Moore; G L Taranger; T O Nilsen; H C Morton; I U Fiksdal; S Stefansson; P G Fjelldal; Ø Evensen; S Patel
Journal:  Virol J       Date:  2016-04-11       Impact factor: 4.099
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