Literature DB >> 6329081

Identification of small DNA fragments synthesized in herpes simplex virus-infected cells in the presence of acyclovir.

P V McGuirt, J E Shaw, G B Elion, P A Furman.   

Abstract

The effect of acyclovir on DNA synthesized in cells infected with herpes simplex virus type 1 was examined. DNA that was synthesized in infected cells in the presence of acyclovir during a short pulse with [3H]thymidine remained near the top of an alkaline sucrose gradient after centrifugation. The sedimentation characteristics of labeled DNA were not changed after chasing in isotope-free medium. The slowly sedimenting DNA was identified as viral in origin by hybridization to purified herpes simplex virus nucleocapsid DNA. When cells were infected with acyclovir-resistant virus containing mutations in the polymerase gene, the viral DNA synthesized in the presence of acyclovir was chased into high-molecular-weight DNA. These findings are consistent with chain termination of herpes simplex virus DNA in virus-infected cells.

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Year:  1984        PMID： 6329081      PMCID： PMC185565          DOI： 10.1128/AAC.25.4.507

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  7 in total

1.  Thymidine kinase from herpes simplex virus phosphorylates the new antiviral compound, 9-(2-hydroxyethoxymethyl)guanine.

Authors:  J A Fyfe; P M Keller; P A Furman; R L Miller; G B Elion
Journal:  J Biol Chem       Date:  1978-12-25       Impact factor: 5.157

2.  Selectivity of action of an antiherpetic agent, 9-(2-hydroxyethoxymethyl) guanine.

Authors:  G B Elion; P A Furman; J A Fyfe; P de Miranda; L Beauchamp; H J Schaeffer
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

3.  Inhibition by acyclovir of cell growth and DNA synthesis of cells biochemically transformed with herpesvirus genetic information.

Authors:  P A Furman; P V McGuirt; P M Keller; J A Fyfe; G B Elion
Journal:  Virology       Date:  1980-04-30       Impact factor: 3.616

4.  Inhibition of herpes simplex virus-induced DNA polymerase activity and viral DNA replication by 9-(2-hydroxyethoxymethyl)guanine and its triphosphate.

Authors:  P A Furman; M H St Clair; J A Fyfe; J L Rideout; P M Keller; G B Elion
Journal:  J Virol       Date:  1979-10       Impact factor: 5.103

5.  Acyclovir inhibition of Epstein-Barr virus replication.

Authors:  A K Datta; B M Colby; J E Shaw; J S Pagano
Journal:  Proc Natl Acad Sci U S A       Date:  1980-09       Impact factor: 11.205

6.  Size, composition, and structure of the deoxyribonucleic acid of herpes simplex virus subtypes 1 and 2.

Authors:  E D Kieff; S L Bachenheimer; B Roizman
Journal:  J Virol       Date:  1971-08       Impact factor: 5.103

7.  Inhibition of purified human and herpes simplex virus-induced DNA polymerases by 9-(2-hydroxyethoxymethyl)guanine triphosphate. Effects on primer-template function.

Authors:  D Derse; Y C Cheng; P A Furman; M H St Clair; G B Elion
Journal:  J Biol Chem       Date:  1981-11-25       Impact factor: 5.157
  7 in total
  13 in total

1.  Nobel lecture in physiology or medicine--1988. The purine path to chemotherapy.

Authors:  G B Elion
Journal:  In Vitro Cell Dev Biol       Date:  1989-04

2.  The enzymological basis for resistance of herpesvirus DNA polymerase mutants to acyclovir: relationship to the structure of alpha-like DNA polymerases.

Authors:  L Huang; K K Ishii; H Zuccola; A M Gehring; C B Hwang; J Hogle; D M Coen
Journal:  Proc Natl Acad Sci U S A       Date:  1999-01-19       Impact factor: 11.205

3.  Inhibition by ganciclovir of cell growth and DNA synthesis of cells biochemically transformed with herpesvirus genetic information.

Authors:  M H St Clair; C U Lambe; P A Furman
Journal:  Antimicrob Agents Chemother       Date:  1987-06       Impact factor: 5.191

4.  Improved DNA hybridization method for detection of acyclovir-resistant herpes simplex virus.

Authors:  E M Swierkosz; D R Scholl; J L Brown; J D Jollick; C A Gleaves
Journal:  Antimicrob Agents Chemother       Date:  1987-10       Impact factor: 5.191

5.  Inhibition of cellular DNA polymerase alpha and human cytomegalovirus-induced DNA polymerase by the triphosphates of 9-(2-hydroxyethoxymethyl)guanine and 9-(1,3-dihydroxy-2-propoxymethyl)guanine.

Authors:  E C Mar; J F Chiou; Y C Cheng; E S Huang
Journal:  J Virol       Date:  1985-03       Impact factor: 5.103

6.  Herpes simplex virus type 1 prereplicative sites are a heterogeneous population: only a subset are likely to be precursors to replication compartments.

Authors:  C J Lukonis; J Burkham; S K Weller
Journal:  J Virol       Date:  1997-06       Impact factor: 5.103

7.  Plaque autoradiography assay for the detection and quantitation of thymidine kinase-deficient and thymidine kinase-altered mutants of herpes simplex virus in clinical isolates.

Authors:  J L Martin; M N Ellis; P M Keller; K K Biron; S N Lehrman; D W Barry; P A Furman
Journal:  Antimicrob Agents Chemother       Date:  1985-08       Impact factor: 5.191

8.  Intranuclear accumulation of subgenomic noninfectious human cytomegalovirus DNA in infected cells in the presence of ganciclovir.

Authors:  F M Hamzeh; P S Lietman
Journal:  Antimicrob Agents Chemother       Date:  1991-09       Impact factor: 5.191

9.  Inhibition of human cytomegalovirus replication by 9-(1,3-dihydroxy-2-propoxymethyl)guanine alone and in combination with human interferons.

Authors:  L Rasmussen; P T Chen; J G Mullenax; T C Merigan
Journal:  Antimicrob Agents Chemother       Date:  1984-10       Impact factor: 5.191

10.  Evaluation of the polymerase chain reaction for diagnosis of herpes simplex virus encephalitis.

Authors:  E Puchhammer-Stöckl; F X Heinz; M Kundi; T Popow-Kraupp; G Grimm; M M Millner; C Kunz
Journal:  J Clin Microbiol       Date:  1993-01       Impact factor: 5.948
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