Effects of deletions on expression of the herpes simplex virus thymidine kinase gene from the intact viral genome: the amino terminus of the enzyme is dispensable for catalytic activity.

We have transferred two deletions affecting the 5' end of the herpes simplex virus thymidine kinase (TK) gene into the intact viral genome. One, extending from -12 to +189, had no effect on TK mRNA synthesis and only a small effect on TK activity, although the first 27 codons of the TK polypeptide were deleted. The other, extending from -85 to +85, severely impaired TK mRNA synthesis. We conclude that the amino terminus of the TK polypeptide is dispensable for catalytic activity, and that expression of TK in viral infections requires some of the same promoter elements used in uninfected cells.