Literature DB >> 6327655

Cloning and characterization of the Bacillus licheniformis gene coding for alkaline phosphatase.

F M Hulett.   

Abstract

The structural gene for alkaline phosphatase (orthophosphoric monoester phosphohydrolase; EC 3.1.3.1) of Bacillus licheniformis MC14 was cloned into the Pst1 site of pMK2004 from chromosomal DNA. The gene was cloned on an 8.5-kilobase DNA fragment. A restriction map was developed, and the gene was subcloned on a 4.2-kilobase DNA fragment. The minimum coding region of the gene was localized to a 1.3-kilobase region. Western blot analysis was used to show that the gene coded for a 60,000-molecular-weight protein which cross-reacts with anti-alkaline phosphatase prepared against the salt-extractable membrane alkaline phosphatase of B. licheniformis MC14 .

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Year:  1984        PMID： 6327655      PMCID： PMC215538          DOI： 10.1128/jb.158.3.978-982.1984

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  26 in total

Review 1.  The assembly of proteins into biological membranes: The membrane trigger hypothesis.

Authors:  W Wickner
Journal:  Annu Rev Biochem       Date:  1979       Impact factor: 23.643

2.  Cloning and restriction mapping of the alkaline phosphatase structural gene (phoA) of Escherichia coli and generation of deletion mutants in vitro.

Authors:  H Inouye; S Michaelis; A Wright; J Beckwith
Journal:  J Bacteriol       Date:  1981-05       Impact factor: 3.490

Review 3.  Mechanisms of protein localization.

Authors:  T J Silhavy; S A Benson; S D Emr
Journal:  Microbiol Rev       Date:  1983-09

4.  Electron microscope histochemical localization of alkaline phosphatase(s) in Bacillus licheniformis.

Authors:  J M McNicholas; F M Hulett
Journal:  J Bacteriol       Date:  1977-01       Impact factor: 3.490

5.  Membrane-associated alkaline phosphatase from Bacillus licheniformis that requires detergent for solubilization: lactoperoxidase 125I localization and molecular weight determination.

Authors:  D B Spencer; J G Hansa; K V Stuckmann; F M Hulett
Journal:  J Bacteriol       Date:  1982-05       Impact factor: 3.490

6.  Intracellular protein topogenesis.

Authors:  G Blobel
Journal:  Proc Natl Acad Sci U S A       Date:  1980-03       Impact factor: 11.205

7.  Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

Authors:  S N Cohen; A C Chang; L Hsu
Journal:  Proc Natl Acad Sci U S A       Date:  1972-08       Impact factor: 11.205

8.  Peptide mapping of Bacillus subtilis RNA polymerase alpha factors and core-associated polypeptides.

Authors:  S L Wong; R H Doi
Journal:  J Biol Chem       Date:  1982-10-25       Impact factor: 5.157

9.  A soluble alkaline phosphatase from Bacillus licheniformis MC14. Histochemical localization, purification, characterization and comparison with the membrane-associated alkaline phosphatase.

Authors:  J G Hansa; M Laporta; M A Kuna; R Reimschuessel; F M Hulett
Journal:  Biochim Biophys Acta       Date:  1981-02-13

10.  Lactoperoxidase-125I localization of salt-extractable alkaline phosphatase on the cytoplasmic membrane of Bacillus licheniformis.

Authors:  D B Spencer; F M Hulett
Journal:  J Bacteriol       Date:  1981-02       Impact factor: 3.490
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  3 in total

Review 1.  Revised genetic linkage map of Bacillus subtilis.

Authors:  P J Piggot; J A Hoch
Journal:  Microbiol Rev       Date:  1985-06

2.  Characterization of the Pseudomonas aeruginosa recA analog and its protein product: rec-102 is a mutant allele of the P. aeruginosa PAO recA gene.

Authors:  T A Kokjohn; R V Miller
Journal:  J Bacteriol       Date:  1987-04       Impact factor: 3.490

3.  Two alkaline phosphatase genes positioned in tandem in Bacillus licheniformis MC14 require different RNA polymerase holoenzymes for transcription.

Authors:  F M Hulett; P Z Wang; M Sussman; J W Lee
Journal:  Proc Natl Acad Sci U S A       Date:  1985-02       Impact factor: 11.205
  3 in total

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