Literature DB >> 6327302

A nuclear-Overhauser-enhancement study of the solution structure of a double-stranded DNA undecamer comprising a portion of the specific target site for the cyclic-AMP-receptor protein in the gal operon. Sequential resonance assignment.

G M Clore, A M Gronenborn.   

Abstract

A 500-MHz 1H-NMR study on a double-stranded non-self-complementary DNA undecamer comprising a portion of the specific target site for the cyclic AMP receptor protein in the gal operon is presented. Using pre-steady-state nuclear Overhauser effect (NOE) measurements, all exchangeable imino, non-exchangeable base, methyl, and H1', H2' and H2" sugar proton resonances are assigned in a sequential manner. In addition, some of the H3' sugar proton resonances are also assigned and some of the exchangeable amino proton resonances identified. The relative magnitudes of the intranucleotide and internucleotide NOEs are indicative of a right-handed B-type conformation for the duplex undecamer in solution.

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Year:  1984        PMID： 6327302     DOI： 10.1111/j.1432-1033.1984.tb08166.x

Source DB:  PubMed          Journal:  Eur J Biochem        ISSN： 0014-2956


  10 in total

1.  Resonance assignments of non-exchangeable protons in B type DNA oligomers, an overview.

Authors:  F J van de Ven; C W Hilbers
Journal:  Nucleic Acids Res       Date:  1988-07-11       Impact factor: 16.971

2.  The solution structure of a RNA pentadecamer comprising the anticodon loop and stem of yeast tRNAPhe. A 500 MHz 1H-n.m.r. study.

Authors:  G M Clore; A M Gronenborn; E A Piper; L W McLaughlin; E Graeser; J H van Boom
Journal:  Biochem J       Date:  1984-08-01       Impact factor: 3.857

3.  An investigation into the solution structure of the single-stranded DNA undecamer 5'd AAGTGTGATAT by means of nuclear Overhauser enhancement measurements.

Authors:  G M Clore; A M Gronenborn
Journal:  Eur Biophys J       Date:  1984       Impact factor: 1.733

4.  Overproduction of the cyclic AMP receptor protein of Escherichia coli and expression of the engineered C-terminal DNA-binding domain.

Authors:  A M Gronenborn; G M Clore
Journal:  Biochem J       Date:  1986-06-15       Impact factor: 3.857

5.  Influence of uracil defect on DNA structure: 1H NMR investigation at 500 MHz.

Authors:  A M Delort; J M Neumann; D Molko; M Hervé; R Téoule; S Tran Dinh
Journal:  Nucleic Acids Res       Date:  1985-05-10       Impact factor: 16.971

6.  1H NMR study of the interaction of bacteriophage lambda Cro protein with the OR3 operator. II. Assignment of the non-exchangeable proton resonances of the OR3 operator.

Authors:  K D Hahn; F Buck; H Rüterjans; B K Chernov; K G Skryabin; M P Kirpichnikov
Journal:  Eur Biophys J       Date:  1985       Impact factor: 1.733

7.  NMR, CD and IR spectroscopies of a tridecanucleotide containing a no-base residue: coexistence of B and Z conformations.

Authors:  S Pochet; T Huynh-Dinh; J M Neumann; S Tran-Dinh; S Adam; J Taboury; E Taillandier; J Igolen
Journal:  Nucleic Acids Res       Date:  1986-01-24       Impact factor: 16.971

8.  An investigation into the solution structures of two self-complementary DNA oligomers, 5'-d(C-G-T-A-C-G) and 5'-d(A-C-G-C-G-C-G-T), by means of nuclear-Overhauser-enhancement measurements.

Authors:  A M Gronenborn; G M Clore; B J Kimber
Journal:  Biochem J       Date:  1984-08-01       Impact factor: 3.857

9.  Helix opening in deoxyribonucleic acid from a proton nuclear magnetic resonance study of imino and amino protons in d(CG)3.

Authors:  G V Fazakerley; G A van der Marel; J H van Boom; W Guschlbauer
Journal:  Nucleic Acids Res       Date:  1984-11-12       Impact factor: 16.971

10.  The solution structure of a B-DNA undecamer comprising a portion of the specific target site for the cAMP receptor protein in the gal operon. Refinement on the basis of interproton distance data.

Authors:  G M Clore; A M Gronenborn
Journal:  EMBO J       Date:  1985-03       Impact factor: 11.598
  10 in total

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