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Literature DB >> 6327261

Isolation and characterization of the rat tryptophan oxygenase gene.

W Schmid, G Scherer, U Danesch, H Zentgraf, P Matthias, C M Strange, W Röwekamp, G Schütz.

Abstract

Tryptophan oxygenase (TO, EC 1.13.1.12) from rat liver is subject to glucocorticoid and developmental control. To study the mechanism of regulation, TO mRNA sequences and the chromosomal TO gene were cloned. From a cDNA library prepared from rat liver poly(A)+ RNA enriched for TO mRNA, a recombinant plasmid containing TO cDNA sequences was identified by translation of hybrid-selected RNA and immunoprecipitation with antibodies directed against TO. This cDNA clone hybridizes to a mRNA 2000 bases long that is inducible by dexamethasone. With this clone as probe we isolated from a bacteriophage lambda rat DNA library genomic clones which together span a region of 32 kilobase pairs (kb). Heteroduplex analysis revealed that the gene extends over 19 kb and is interrupted by at least 11 introns. To characterize the presumptive control region the DNA sequence around the 5' end of the TO gene was determined. S1 nuclease protection experiments revealed two separate start sites for TO mRNA transcription within this region.

Entities: Chemical

Mesh：

Substances：

Year: 1982 PMID： 6327261 PMCID： PMC553202 DOI： 10.1002/j.1460-2075.1982.tb00026.x

Source DB: PubMed Journal: EMBO J ISSN： 0261-4189 Impact factor: 11.598

45 in total

1. Screening lambdagt recombinant clones by hybridization to single plaques in situ.

Authors: W D Benton; R W Davis
Journal: Science Date: 1977-04-08 Impact factor: 47.728

2. Messenger RNA for hepatic tryptophan oxygenase: its partial purification, its translation in a heterologous cell-free system, and its control by glucocorticoid hormones.

Authors: G Schutz; M Beato; P Feigelson
Journal: Proc Natl Acad Sci U S A Date: 1973-04 Impact factor: 11.205

3. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.

Authors: W M Bonner; R A Laskey
Journal: Eur J Biochem Date: 1974-07-01

4. The effect of development and hydrocortisone on tryptophan oxygenase, formamidase, and tyrosine aminotransferase in the livers of young rats.

Authors: J M Franz; W E Knox
Journal: Biochemistry Date: 1967-11 Impact factor: 3.162

5. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

6. Nucleotide sequence of cloned rat serum albumin messenger RNA.

Authors: T D Sargent; M Yang; J Bonner
Journal: Proc Natl Acad Sci U S A Date: 1981-01 Impact factor: 11.205

7. Translation of mRNA from rat-liver polysomes into tyrosine aminotransferase and tryptophan oxygenase in a protein-synthesizing system from wheat germ. Effects of cortisol on the translatable levels of mRNA for these two enzymes.

Authors: W G Roewekamp; E Hofer; C E Sekeris
Journal: Eur J Biochem Date: 1976-11-01

8. Pretranslational control of tryptophan oxygenase levels in Morris hepatoma and host liver.

Authors: L Ramanarayanan-Murthy; P D Colman; H P Morris; P Feigelson
Journal: Cancer Res Date: 1976-10 Impact factor: 12.701

9. Thyroid or glucocorticoid hormone induces pre-growth-hormone mRNA and its probable nuclear precursor in rat pituitary cells.

Authors: P R Dobner; E S Kawasaki; L Y Yu; F C Bancroft
Journal: Proc Natl Acad Sci U S A Date: 1981-04 Impact factor: 11.205

10. Control of the mRNA for hepatic tryptophan oxygenase during hormonal and substrate induction.

Authors: G Schutz; L Killewich; G Chen; P Feigelson
Journal: Proc Natl Acad Sci U S A Date: 1975-03 Impact factor: 11.205

15 in total

1. GATA4 inhibits expression of the tryptophan oxygenase gene by binding to the TATA box in fetal hepatocytes.

Authors: Hidenori Kaneoka; Katsuhide Miyake; Shinji Iijima
Journal: Cytotechnology Date: 2008-01-11 Impact factor: 2.058

2. Characterization of the 5'-flanking region for the human fibrinogen beta gene.

Authors: P Huber; J Dalmon; G Courtois; M Laurent; Z Assouline; G Marguerie
Journal: Nucleic Acids Res Date: 1987-02-25 Impact factor: 16.971

3. Identification of the promoter sequences involved in the interleukin-6 dependent expression of the rat alpha 2-macroglobulin gene.

Authors: D Kunz; R Zimmermann; M Heisig; P C Heinrich
Journal: Nucleic Acids Res Date: 1989-02-11 Impact factor: 16.971

Isolation and characterization of the rat tryptophan oxygenase gene.

1. Screening lambdagt recombinant clones by hybridization to single plaques in situ.

2. Messenger RNA for hepatic tryptophan oxygenase: its partial purification, its translation in a heterologous cell-free system, and its control by glucocorticoid hormones.

3. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.

4. The effect of development and hydrocortisone on tryptophan oxygenase, formamidase, and tyrosine aminotransferase in the livers of young rats.

5. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

6. Nucleotide sequence of cloned rat serum albumin messenger RNA.

7. Translation of mRNA from rat-liver polysomes into tyrosine aminotransferase and tryptophan oxygenase in a protein-synthesizing system from wheat germ. Effects of cortisol on the translatable levels of mRNA for these two enzymes.

8. Pretranslational control of tryptophan oxygenase levels in Morris hepatoma and host liver.

9. Thyroid or glucocorticoid hormone induces pre-growth-hormone mRNA and its probable nuclear precursor in rat pituitary cells.

10. Control of the mRNA for hepatic tryptophan oxygenase during hormonal and substrate induction.

1. GATA4 inhibits expression of the tryptophan oxygenase gene by binding to the TATA box in fetal hepatocytes.

2. Characterization of the 5'-flanking region for the human fibrinogen beta gene.

3. Identification of the promoter sequences involved in the interleukin-6 dependent expression of the rat alpha 2-macroglobulin gene.

4. Isolation and characterization of the rat tyrosine aminotransferase gene.

Review 5. Applications of recombinant DNA technology to studies of metabolic regulation.

6. The uteroglobin gene region: hormonal regulation, repetitive elements and complete nucleotide sequence of the gene.

7. The first intron of the human growth hormone gene contains a binding site for glucocorticoid receptor.

8. Point mutations in the tyrosine aminotransferase gene in tyrosinemia type II.

9. Transcriptional activation of the rat liver tyrosine aminotransferase gene by cAMP.

10. Post-transcriptional regulation of rat liver gene expression by glucocorticoids.