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Literature DB >> 6326754

Measurement of intrasynaptosomal free calcium by using the fluorescent indicator quin-2.

Abstract

The recently synthesized calcium indicator quin -2 was incorporated into synaptosomes from guinea-pig cerebral cortex following uptake and internal hydrolysis of quin -2 tetra-acetoxymethyl ester. Incubation in physiological media containing 1 mM- or 2 mM-CaCl2 led to equilibrium cytosolic ionized calcium concentrations of 85 +/- 10 nM and 205 +/- 5 nM respectively (mean +/- S.E.M. from eight and eighteen preparations respectively). Cytosolic Ca2+ was elevated following increases in external Ca2+ concentration, plasma membrane depolarization, mitochondrial inhibition, calcium ionophore addition or replacement of external sodium by lithium. Preliminary experiments were performed to assess changes in cytosolic Ca2+ accompanying the release of the neurotransmitter acetylcholine.

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Year: 1984 PMID： 6326754 PMCID： PMC1153459 DOI： 10.1042/bj2190149

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

27 in total

1. The isolation of nerve endings from brain: an electron-microscopic study of cell fragments derived by homogenization and centrifugation.

Authors: E G GRAY; V P WHITTAKER
Journal: J Anat Date: 1962-01 Impact factor: 2.610

2. Coupling of phosphorylation to electron and hydrogen transfer by a chemi-osmotic type of mechanism.

Authors: P MITCHELL
Journal: Nature Date: 1961-07-08 Impact factor: 49.962

Review 3. Depolarization and excitation-secretion coupling in presynaptic terminals.

Authors: P W Gage
Journal: Fed Proc Date: 1967 Nov-Dec

4. Evoked transient intracellular free Ca2+ changes and secretion in isolated bovine adrenal medullary cells.

Authors: D E Knight; N T Kesteven
Journal: Proc R Soc Lond B Biol Sci Date: 1983-05-23

5. The permeability of pinched-off nerve endings to sodium, potassium and chloride and the effects of gramicidin.

Authors: P Keen; T D White
Journal: J Neurochem Date: 1971-06 Impact factor: 5.372

6. The effect of ouabain on the release of [14C]acetylcholine and other substances from synaptosomes.

Authors: S Vyas; R M Marchbanks
Journal: J Neurochem Date: 1981-12 Impact factor: 5.372

Review 7. Calcium channels in excitable cell membranes.

Authors: R W Tsien
Journal: Annu Rev Physiol Date: 1983 Impact factor: 19.318

8. Transmitter release induced by injection of calcium ions into nerve terminals.

Authors: R Miledi
Journal: Proc R Soc Lond B Biol Sci Date: 1973-07-03

9. The osmotically sensitive potassium and sodium compartments of synaptosomes.

Authors: R M Marchbanks
Journal: Biochem J Date: 1967-07 Impact factor: 3.857

10. A calcium ionophore simulating the action of epinephrine on the alpha-adrenergic receptor.

Authors: Z Selinger; S Eimerl; M Schramm
Journal: Proc Natl Acad Sci U S A Date: 1974-01 Impact factor: 11.205

19 in total

1. Exacerbated responses to oxidative stress by an Na(+) load in isolated nerve terminals: the role of ATP depletion and rise of [Ca(2+)](i).

Authors: C Chinopoulos; L Tretter; A Rozsa; V Adam-Vizi
Journal: J Neurosci Date: 2000-03-15 Impact factor: 6.167

6. Lack of excitatory amino acid-induced effects on calcium fluxes measured with 45Ca2+ in rat cerebral cortex synaptosomes.

Authors: M Simonato; R S Jope; C Bianchi; L Beani
Journal: Neurochem Res Date: 1989-07 Impact factor: 3.996

Measurement of intrasynaptosomal free calcium by using the fluorescent indicator quin-2.

1. The isolation of nerve endings from brain: an electron-microscopic study of cell fragments derived by homogenization and centrifugation.

2. Coupling of phosphorylation to electron and hydrogen transfer by a chemi-osmotic type of mechanism.

Review 3. Depolarization and excitation-secretion coupling in presynaptic terminals.

4. Evoked transient intracellular free Ca2+ changes and secretion in isolated bovine adrenal medullary cells.

5. The permeability of pinched-off nerve endings to sodium, potassium and chloride and the effects of gramicidin.

6. The effect of ouabain on the release of [14C]acetylcholine and other substances from synaptosomes.

Review 7. Calcium channels in excitable cell membranes.

8. Transmitter release induced by injection of calcium ions into nerve terminals.

9. The osmotically sensitive potassium and sodium compartments of synaptosomes.

10. A calcium ionophore simulating the action of epinephrine on the alpha-adrenergic receptor.

1. Exacerbated responses to oxidative stress by an Na(+) load in isolated nerve terminals: the role of ATP depletion and rise of [Ca(2+)](i).

2. Regulation of cytosolic free calcium concentration by intrasynaptic mitochondria.

3. Cytosolic free calcium concentrations in synaptosomes during histotoxic hypoxia.

Review 4. Dehydrogenase activation by Ca2+ in cells and tissues.

5. Role of Ca2+ in pyruvate dehydrogenase interconversion in brain mitochondria and synaptosomes.

6. Lack of excitatory amino acid-induced effects on calcium fluxes measured with 45Ca2+ in rat cerebral cortex synaptosomes.

7. The action of volatile anaesthetics on stimulus-secretion coupling in bovine adrenal chromaffin cells.

8. Calcium efflux and cycling across the synaptosomal plasma membrane.

9. Proceedings of the British Pharmacological Society. 18th-20th December 1985. Abstracts.

10. Menadione-treated synaptosomes as a model for post-ischaemic neuronal damage.