Literature DB >> 6326623

Use of differential dye-ligand chromatography with affinity elution for enzyme purification: 6-phosphogluconate dehydratase from Zymomonas mobilis.

R K Scopes, K Griffiths-Smith.   

Abstract

Using differential dye-ligand chromatography and affinity elution with a substrate analog, 6-phosphogluconate dehydratase (EC 4.2.1.12) has been isolated from extracts of Zymomonas mobilis in a one-step procedure with 50% recovery. The specific activity of freshly isolated enzyme was 245 units mg-1. The enzyme contains iron, and it is rapidly inactivated in oxidizing conditions. It is inhibited by glycerophosphates, most strongly by the D-alpha-isomer which structurally corresponds to half of the substrate molecule.

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Year:  1984        PMID： 6326623     DOI： 10.1016/0003-2697(84)90257-4

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  13 in total

1.  Immunocytochemical localization of glycolytic and fermentative enzymes in Zymomonas mobilis.

Authors:  H C Aldrich; L McDowell; M F Barbosa; L P Yomano; R K Scopes; L O Ingram
Journal:  J Bacteriol       Date:  1992-07       Impact factor: 3.490

2.  The polycistronic mRNA of the Zymomonas mobilis glf-zwf-edd-glk operon is subject to complex transcript processing.

Authors:  J Liu; W O Barnell; T Conway
Journal:  J Bacteriol       Date:  1992-05       Impact factor: 3.490

3.  Gel electrophoretic analysis of Zymomonas mobilis glycolytic and fermentative enzymes: identification of alcohol dehydrogenase II as a stress protein.

Authors:  H An; R K Scopes; M Rodriguez; K F Keshav; L O Ingram
Journal:  J Bacteriol       Date:  1991-10       Impact factor: 3.490

4.  Simultaneous purification and characterization of glucokinase, fructokinase and glucose-6-phosphate dehydrogenase from Zymomonas mobilis.

Authors:  R K Scopes; V Testolin; A Stoter; K Griffiths-Smith; E M Algar
Journal:  Biochem J       Date:  1985-06-15       Impact factor: 3.857

5.  Isolation and properties of the glycolytic enzymes from Zymomonas mobilis. The five enzymes from glyceraldehyde-3-phosphate dehydrogenase through to pyruvate kinase.

Authors:  A Pawluk; R K Scopes; K Griffiths-Smith
Journal:  Biochem J       Date:  1986-08-15       Impact factor: 3.857

6.  The Zymomonas mobilis glf, zwf, edd, and glk genes form an operon: localization of the promoter and identification of a conserved sequence in the regulatory region.

Authors:  W O Barnell; J Liu; T L Hesman; M C O'Neill; T Conway
Journal:  J Bacteriol       Date:  1992-05       Impact factor: 3.490

7.  Sequence and genetic organization of a Zymomonas mobilis gene cluster that encodes several enzymes of glucose metabolism.

Authors:  W O Barnell; K C Yi; T Conway
Journal:  J Bacteriol       Date:  1990-12       Impact factor: 3.490

8.  Glycolytic flux in Zymomonas mobilis: enzyme and metabolite levels during batch fermentation.

Authors:  Y A Osman; T Conway; S J Bonetti; L O Ingram
Journal:  J Bacteriol       Date:  1987-08       Impact factor: 3.490

9.  Coordination of expression of Zymomonas mobilis glycolytic and fermentative enzymes: a simple hypothesis based on mRNA stability.

Authors:  J P Mejia; M E Burnett; H An; W O Barnell; K F Keshav; T Conway; L O Ingram
Journal:  J Bacteriol       Date:  1992-10       Impact factor: 3.490

10.  Two genes for carbohydrate catabolism are divergently transcribed from a region of DNA containing the hexC locus in Pseudomonas aeruginosa PAO1.

Authors:  L Temple; A Sage; G E Christie; P V Phibbs
Journal:  J Bacteriol       Date:  1994-08       Impact factor: 3.490
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