Literature DB >> 6325247

Purification and properties of suppressor seryl-tRNA: ATP phosphotransferase from bovine liver.

T Mizutani, A Hashimoto.   

Abstract

Seryl-tRNASerCmCA: ATP phosphotransferase was purified 1200-fold from bovine liver by ultracentrifugation at 150 000 X g, chromatography on DEAE-cellulose, fractional precipitation with ammonium sulfate, chromatography on hydroxyapatite, gel filtration on Sephacryl S-300 and affinity chromatography on Blue Sepharose. Molecular mass was estimated as 135-145 kDa. The Km values for ATP and ser-tRNASerCmCA were 2 mM and 21 nM, respectively. This enzyme did not react with ser-tRNASerIGA, tyr-tRNA or thr-tRNA.

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Year:  1984        PMID： 6325247     DOI： 10.1016/0014-5793(84)80342-7

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  15 in total

1.  pGp as the main product of bovine tRNA kinase.

Authors:  Takaharu Mizutani; Takashi Osaka; Yuko Ito; Masanobu Kanou; Toru Usui; Yumiko Sone; Tsuyoshi Totsuka
Journal:  Mol Biol Rep       Date:  2002-09       Impact factor: 2.316

2.  Unique pathway of expression of an opal suppressor phosphoserine tRNA.

Authors:  B J Lee; P de la Peña; J A Tobian; M Zasloff; D Hatfield
Journal:  Proc Natl Acad Sci U S A       Date:  1987-09       Impact factor: 11.205

3.  The high content of natural suppressor serine tRNA in dystrophic mouse muscle.

Authors:  T Hitaka; T Mizutani; K Watanabe; T Totsuka
Journal:  Biochem J       Date:  1990-02-15       Impact factor: 3.857

4.  Selenium induces changes in the selenocysteine tRNA[Ser]Sec population in mammalian cells.

Authors:  D Hatfield; B J Lee; L Hampton; A M Diamond
Journal:  Nucleic Acids Res       Date:  1991-02-25       Impact factor: 16.971

5.  Modulation of glutathione peroxidase expression by selenium: effect on human MCF-7 breast cancer cell transfectants expressing a cellular glutathione peroxidase cDNA and doxorubicin-resistant MCF-7 cells.

Authors:  F F Chu; R S Esworthy; S Akman; J H Doroshow
Journal:  Nucleic Acids Res       Date:  1990-03-25       Impact factor: 16.971

6.  Selenocysteine tRNA[Ser]Sec gene is ubiquitous within the animal kingdom.

Authors:  B J Lee; M Rajagopalan; Y S Kim; K H You; K B Jacobson; D Hatfield
Journal:  Mol Cell Biol       Date:  1990-05       Impact factor: 4.272

7.  Some properties of murine selenocysteine synthase.

Authors:  T Mizutani; H Kurata; K Yamada; T Totsuka
Journal:  Biochem J       Date:  1992-06-15       Impact factor: 3.857

8.  Eukaryotic selenocysteine inserting tRNA species support selenoprotein synthesis in Escherichia coli.

Authors:  C Baron; C Sturchler; X Q Wu; H J Gross; A Krol; A Böck
Journal:  Nucleic Acids Res       Date:  1994-06-25       Impact factor: 16.971

9.  The dual identities of mammalian tRNA(Sec) for SerRS and selenocysteine synthase.

Authors:  T Mizutani; K Kanaya; S Ikeda; T Fujiwara; K Yamada; T Totsuka
Journal:  Mol Biol Rep       Date:  1998-11       Impact factor: 2.316

10.  Conserved sequences in both coding and 5' flanking regions of mammalian opal suppressor tRNA genes.

Authors:  K Pratt; F C Eden; K H You; V A O'Neill; D Hatfield
Journal:  Nucleic Acids Res       Date:  1985-07-11       Impact factor: 16.971
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