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Literature DB >> 6324794

Proline hydroxylation by cell free extract of a streptomycete.

M Onishi, Y Okumura, R Okamoto, T Ishikura.

Abstract

Free L-proline was hydroxylated to free L-hydroxyproline by cell free extract of Streptomyces griseoviridus P8648. The hydroxylation reaction required ferrous ion, 2-ketoglutarate and ascorbate. Zinc ion, ethylenediaminetetraacetic acid and alpha,alpha'-dipyridyl inhibited the reaction. Optimum temperature and pH were 25.0 degrees C and 7.5, respectively.

Entities: Chemical

Mesh：

Substances：

Year: 1984 PMID： 6324794 DOI： 10.1016/0006-291x(84)91411-6

Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575

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Cited

8 in total

1. Purification and cloning of a proline 3-hydroxylase, a novel enzyme which hydroxylates free L-proline to cis-3-hydroxy-L-proline.

Authors: H Mori; T Shibasaki; K Yano; A Ozaki
Journal: J Bacteriol Date: 1997-09 Impact factor: 3.490

2. Microbial proline 4-hydroxylase screening and gene cloning.

Authors: T Shibasaki; H Mori; S Chiba; A Ozaki
Journal: Appl Environ Microbiol Date: 1999-09 Impact factor: 4.792

3. Detection of Novel Proline 3-Hydroxylase Activities in Streptomyces and Bacillus spp. by Regio- and Stereospecific Hydroxylation of l-Proline.

Authors: H Mori; T Shibasaki; Y Uozaki; K Ochiai; A Ozaki
Journal: Appl Environ Microbiol Date: 1996-06 Impact factor: 4.792

4. Purification and initial characterization of proline 4-hydroxylase from Streptomyces griseoviridus P8648: a 2-oxoacid, ferrous-dependent dioxygenase involved in etamycin biosynthesis.

Authors: C C Lawrence; W J Sobey; R A Field; J E Baldwin; C J Schofield
Journal: Biochem J Date: 1996-01-01 Impact factor: 3.857

Proline hydroxylation by cell free extract of a streptomycete.

1. Purification and cloning of a proline 3-hydroxylase, a novel enzyme which hydroxylates free L-proline to cis-3-hydroxy-L-proline.

2. Microbial proline 4-hydroxylase screening and gene cloning.

3. Detection of Novel Proline 3-Hydroxylase Activities in Streptomyces and Bacillus spp. by Regio- and Stereospecific Hydroxylation of l-Proline.

4. Purification and initial characterization of proline 4-hydroxylase from Streptomyces griseoviridus P8648: a 2-oxoacid, ferrous-dependent dioxygenase involved in etamycin biosynthesis.

5. Enzymatic production of trans-4-hydroxy-l-proline by proline 4-hydroxylase.

6. Studies on the selectivity of proline hydroxylases reveal new substrates including bicycles.

Review 7. Metabolic engineering strategy for synthetizing trans-4-hydroxy-L-proline in microorganisms.

8. Biosynthesis of trans-4-hydroxyproline by recombinant strains of Corynebacterium glutamicum and Escherichia coli.