Literature DB >> 6324762

A problem in the radiochemical assay of glucose-6-phosphatase in muscle.

R Lackner, R A Challiss, D West, E A Newsholme.   

Abstract

A radiochemical assay for glucose-6-phosphatase, which depends on the absorption of radiolabelled substrate on to a nascent precipitate of BaSO4 and Zn(OH)2, can give rise to erroneously high activities. Adsorption on to a Fe(OH)3 precipitate does not expose the incubation medium to extreme alkaline conditions and provides a satisfactory and simple assay. This method demonstrates a very low activity of the enzyme in mammalian muscle, in contrast with previous findings.

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Year:  1984        PMID： 6324762      PMCID： PMC1153387          DOI： 10.1042/bj2180649

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  7 in total

1.  Acid phosphatase. VI. Kinetic properties of purified yeast and erythrocyte phosphomonoesterase.

Authors:  K K TSUBOI; P B HUDSON
Journal:  Arch Biochem Biophys       Date:  1956-03       Impact factor: 4.013

2.  A method for the determination of glucose-6-phosphatase activity in rat liver with [U-14C]glucose 6-phosphate as substrate.

Authors:  S A Kitcher; K Siddle; J P Luzio
Journal:  Anal Biochem       Date:  1978-07-15       Impact factor: 3.365

3.  Regulation of glucose uptake by muscle. 6. Fructose 1,6-diphosphatase activity of rat heart and rat diaphragm.

Authors:  E A Newsholme; P J Randle
Journal:  Biochem J       Date:  1962-05       Impact factor: 3.857

4.  Activities of citrate synthase and NAD+-linked and NADP+-linked isocitrate dehydrogenase in muscle from vertebrates and invertebrates.

Authors:  P R Alp; E A Newsholme; V A Zammit
Journal:  Biochem J       Date:  1976-03-15       Impact factor: 3.857

5.  Maximum activities and properties of glucose 6-phosphatase in muscles from vertebrates and invertebrates.

Authors:  B Surholt; E A Newsholme
Journal:  Biochem J       Date:  1981-09-15       Impact factor: 3.857

6.  A rapid method for isolating glucose metabolites involved in substrate cycling.

Authors:  R H Hammerstedt
Journal:  Anal Biochem       Date:  1980-12       Impact factor: 3.365

7.  The rate of substrate cycling between glucose and glucose 6-phosphate in muscle and fat-body of the hawk moth (Acherontia atropos) at rest and during flight.

Authors:  B Surholt; E A Newsholme
Journal:  Biochem J       Date:  1983-01-15       Impact factor: 3.857
  7 in total
  6 in total

1.  Exercise restores skeletal muscle glucose delivery but not insulin-mediated glucose transport and phosphorylation in obese subjects.

Authors:  L Slimani; V Oikonen; K Hällsten; N Savisto; J Knuuti; P Nuutila; P Iozzo
Journal:  J Clin Endocrinol Metab       Date:  2006-06-13       Impact factor: 5.958

2.  Glucose uptake and lumped constant variability in normal human hearts determined with [18F]fluorodeoxyglucose.

Authors:  H E Bøtker; M Böttcher; O Schmitz; A Gee; S B Hansen; G E Cold; T T Nielsen; A Gjedde
Journal:  J Nucl Cardiol       Date:  1997 Mar-Apr       Impact factor: 5.952

3.  A method to quantify glucose utilization in vivo in skeletal muscle and white adipose tissue of the anaesthetized rat.

Authors:  P Ferré; A Leturque; A F Burnol; L Penicaud; J Girard
Journal:  Biochem J       Date:  1985-05-15       Impact factor: 3.857

4.  Compartmentation of hexokinase in rat heart. A critical factor for tracer kinetic analysis of myocardial glucose metabolism.

Authors:  R R Russell; J M Mrus; J I Mommessin; H Taegtmeyer
Journal:  J Clin Invest       Date:  1992-11       Impact factor: 14.808

5.  Fuel utilization in colonocytes of the rat.

Authors:  M S Ardawi; E A Newsholme
Journal:  Biochem J       Date:  1985-11-01       Impact factor: 3.857

6.  Effect of a tumour-derived lipid-mobilising factor on glucose and lipid metabolism in vivo.

Authors:  S T Russell; M J Tisdale
Journal:  Br J Cancer       Date:  2002-08-27       Impact factor: 7.640
  6 in total

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