Literature DB >> 6324123

The methylation pattern of tRNA genes in Xenopus laevis.

S Talwar, M J Pocklington, N Maclean.   

Abstract

The methylation sensitive restriction enzymes Hha I and Hpa II were used to analyse the methylation pattern of a tRNA gene cluster in germ line and somatic DNA of Xenopus laevis. A single tDNA repeat containing 8 tRNA genes was studied and all copies were found to be fully modified in sperm DNA. In the DNA from erythroid cells, hepatocytes, kidney and brain, most tDNA repeats were found to be fully modified. However, in a fraction of the repeats, specific demethylated sites can be detected, giving rise to a pattern which does not vary significantly from one tissue to another. Although our results do not allow a straightforward correlation between hypomethylation and tRNA gene transcription, they are in agreement with the observation that hypomethylation accompanies differentiation and development.

Entities:  

Mesh：

Substances：

Year:  1984        PMID： 6324123      PMCID： PMC318680          DOI： 10.1093/nar/12.5.2509

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  22 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

3.  Specific DNA methylation sites in the vicinity of the chicken beta-globin genes.

Authors:  J D McGhee; G D Ginder
Journal:  Nature       Date:  1979-08-02       Impact factor: 49.962

4.  Reiterated transfer RNA genes of Xenopus laevis.

Authors:  S G Clarkson; M L Birnstiel; V Serra
Journal:  J Mol Biol       Date:  1973-09-15       Impact factor: 5.469

5.  The in vivo methylation of DNA in mouse fibroblasts.

Authors:  R H Burdon; R L Adams
Journal:  Biochim Biophys Acta       Date:  1969-01-21

6.  Methylation of DNA in developing sea urchin embryos.

Authors:  P Grippo; M Iaccarino; E Parisi; E Scarano
Journal:  J Mol Biol       Date:  1968-09-14       Impact factor: 5.469

7.  DNA methylation: organ specific variations in the methylation pattern within and around ovalbumin and other chicken genes.

Authors:  J L Mandel; P Chambon
Journal:  Nucleic Acids Res       Date:  1979-12-20       Impact factor: 16.971

8.  DNA methylation: correlation with DNase I sensitivity of chicken ovalbumin and conalbumin chromatin.

Authors:  M T Kuo; J L Mandel; P Chambon
Journal:  Nucleic Acids Res       Date:  1979-12-20       Impact factor: 16.971

9.  Sequence organization of a cloned tDNA met fragment from Xenopus laevis.

Authors:  S G Clarkson; V Kurer; H O Smith
Journal:  Cell       Date:  1978-07       Impact factor: 41.582

10.  The erythroid cells of anaemic Xenopus laevis. I. Studies on cellular morphology and protein and nucleic acid synthesis during differentiation.

Authors:  N Thomas; N Maclean
Journal:  J Cell Sci       Date:  1975-12       Impact factor: 5.285
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.