Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Nucleotide sequence of the Escherichia coli xanthine-guanine phosphoribosyl transferase gene.

Literature DB >> 6324103

Nucleotide sequence of the Escherichia coli xanthine-guanine phosphoribosyl transferase gene.

Abstract

The Escherichia coli gene coding for the enzyme xanthine-guanine phosphoribosyl transferase (gpt) has been widely used as a dominant selectable marker in a variety of mammalian cells. We have determined the complete nucleotide sequence of the 1057 base pair (bp) segment of DNA containing this gene. The coding sequence for the enzyme is 456 nucleotides long and can code for a 152 amino acid (16.9 Kd) polypeptide. A comparison of the amino acid sequence of the bacterial enzyme with that of the mammalian hypoxanthine-guanine phosphoribosyl transferase (hprt) reveals no significant homology between the two polypeptides.

Entities: Gene Species

Mesh：

Substances：

Year: 1983 PMID： 6324103 PMCID： PMC326626 DOI： 10.1093/nar/11.24.8817

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

13 in total

1. Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter.

Authors: D Pribnow
Journal: Proc Natl Acad Sci U S A Date: 1975-03 Impact factor: 11.205

2. 3'-end labeling of DNA with [alpha-32P]cordycepin-5'-triphosphate.

Authors: C P Tu; S N Cohen
Journal: Gene Date: 1980-07 Impact factor: 3.688

3. Inosine and guanine phosphoribosyltransferase in Escherichia coli.

Authors: W R Martin; R R Yang
Journal: Biochem Biophys Res Commun Date: 1972-09-26 Impact factor: 3.575

4. The regulation of purine utilization in bacteria. I. Purification of adenine phosphoribosyltransferase from Escherichia coli K12 and control of activity by nucleotides.

Authors: J Hochstadt-Ozer; E R Stadtman
Journal: J Biol Chem Date: 1971-09-10 Impact factor: 5.157

5. The regulation of purine utilization in bacteria. III. The involvement of purine phosphoribosyltransferases in the uptake of adenine and other nucleic acid precursors by intact resting cells.

Authors: J Hochstadt-Ozer; E R Stadtman
Journal: J Biol Chem Date: 1971-09-10 Impact factor: 5.157

6. Improved estimation of secondary structure in ribonucleic acids.

Authors: I Tinoco; P N Borer; B Dengler; M D Levin; O C Uhlenbeck; D M Crothers; J Bralla
Journal: Nat New Biol Date: 1973-11-14

7. Preparative and analytical purification of DNA from agarose.

Authors: B Vogelstein; D Gillespie
Journal: Proc Natl Acad Sci U S A Date: 1979-02 Impact factor: 11.205

8. Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors: A M Maxam; W Gilbert
Journal: Methods Enzymol Date: 1980 Impact factor: 1.600

9. Escherichia coli mutants deficient in guanine-xanthine phosphoribosyltransferase.

Authors: J A Holden; P D Harriman; J D Wall
Journal: J Bacteriol Date: 1976-06 Impact factor: 3.490

10. Expression of a bacterial gene in mammalian cells.

Authors: R C Mulligan; P Berg
Journal: Science Date: 1980-09-19 Impact factor: 47.728

16 in total

Review 1. Linkage map of Escherichia coli K-12, edition 10: the traditional map.

Authors: M K Berlyn
Journal: Microbiol Mol Biol Rev Date: 1998-09 Impact factor: 11.056

Review 2. Linkage map of Escherichia coli K-12, edition 8.

Authors: B J Bachmann
Journal: Microbiol Rev Date: 1990-06

3. Random isolation of gene activator elements from the human genome.

Authors: H Hamada
Journal: Mol Cell Biol Date: 1986-12 Impact factor: 4.272

4. A novel fluorescence-based system for assaying and separating live cells according to VDJ recombinase activity.

Authors: G D Yancopoulos; G P Nolan; R Pollock; S Prockop; S C Li; L A Herzenberg; F W Alt
Journal: Mol Cell Biol Date: 1990-04 Impact factor: 4.272

5. Preparation of partially 2H/13C-labelled RNA for NMR studies. Stereo-specific deuteration of the H5" in nucleotides.

Authors: Jenny Cromsigt; Jürgen Schleucher; Tomas Gustafsson; Jan Kihlberg; Sybren Wijmenga
Journal: Nucleic Acids Res Date: 2002-04-01 Impact factor: 16.971

6. Sequence analysis of spontaneous mutations in a shuttle vector gene integrated into mammalian chromosomal DNA.

Authors: C R Ashman; R L Davidson
Journal: Proc Natl Acad Sci U S A Date: 1987-05 Impact factor: 11.205

7. Cloning of a guanosine-inosine kinase gene of Escherichia coli and characterization of the purified gene product.

Authors: H Mori; A Iida; S Teshiba; T Fujio
Journal: J Bacteriol Date: 1995-09 Impact factor: 3.490

8. Inactivation of a transfected gene in human fibroblasts can occur by deletion, amplification, phenotypic switching, or methylation.

Authors: M M Gebara; C Drevon; S A Harcourt; H Steingrimsdottir; M R James; J F Burke; C F Arlett; A R Lehmann
Journal: Mol Cell Biol Date: 1987-04 Impact factor: 4.272

9. Transfection of normal human and Chinese hamster DNA corrects diepoxybutane-induced chromosomal hypersensitivity of Fanconi anemia fibroblasts.

Authors: M Shaham; B Adler; S Ganguly; R S Chaganti
Journal: Proc Natl Acad Sci U S A Date: 1987-08 Impact factor: 11.205

10. Analysis of cDNA encoding the hypoxanthine-guanine phosphoribosyltransferase (HGPRTase) of Schistosoma mansoni; a putative target for chemotherapy.

Authors: S P Craig; J H McKerrow; G R Newport; C C Wang
Journal: Nucleic Acids Res Date: 1988-07-25 Impact factor: 16.971