Literature DB >> 6324076

The origin of the rRNA precursor from Xenopus borealis, analysed in vivo and in vitro.

B McStay, A Bird.   

Abstract

We have determined the origin of the major transcript of Xenopus borealis rDNA by the use of an SI nuclease protection assay. The DNA surrounding the origin of this transcript was sequenced, and the region upstream of the origin was shown to have strong sequence homology with that region from X.laevis rDNA. We have also demonstrated faithful transcription from this origin using cloned X.borealis rDNA in an extract derived from X. laevis culture cells. This in vitro transcription was insensitive to 100 micrograms/ml alpha-amanatin, suggesting that it was mediated by RNA polymerase 1.

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Year:  1983        PMID: 6324076      PMCID: PMC326573          DOI: 10.1093/nar/11.23.8167

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN: 0305-1048            Impact factor:   16.971


  33 in total

1.  Expression of human and suppression of mouse nucleolus organizer activity in mouse-human somatic cell hybrids.

Authors:  O J Miller; D A Miller; V G Dev; R Tantravahi; C M Croce
Journal:  Proc Natl Acad Sci U S A       Date:  1976-12       Impact factor: 11.205

2.  Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.

Authors:  A J Berk; P A Sharp
Journal:  Cell       Date:  1977-11       Impact factor: 41.582

3.  Suppression of human nucleolus organizer activity in mouse-human somatic hybrid cells.

Authors:  D A Miller; V G Dev; R Tantravahi; O J Miller
Journal:  Exp Cell Res       Date:  1976-09       Impact factor: 3.905

4.  Sequence organization of the spacer DNA in a ribosomal gene unit of Xenopus laevis.

Authors:  P Boseley; T Moss; M Mächler; R Portmann; M Birnstiel
Journal:  Cell       Date:  1979-05       Impact factor: 41.582

5.  An electron microscope heteroduplex study of the ribosomal DNAs of Xenopus laevis and Xenopus mulleri.

Authors:  A B Forsheit; N Davidson; D D Brown
Journal:  J Mol Biol       Date:  1974-12-05       Impact factor: 5.469

6.  Preferential transcription of Xenopus laevis ribosomal RNA in interspecies hybrids between Xenopus laevis and Xenopus mulleri.

Authors:  T Honjo; R H Reeder
Journal:  J Mol Biol       Date:  1973-10-25       Impact factor: 5.469

7.  A comparison of the ribosomal DNA's of Xenopus laevis and Xenopus mulleri: the evolution of tandem genes.

Authors:  D D Brown; P C Wensink; E Jordan
Journal:  J Mol Biol       Date:  1972-01-14       Impact factor: 5.469

8.  Selective suppression of the transcription of ribosomal genes in mouse-human hybrid cells.

Authors:  R P Perry; D E Kelley; U Schibler; K Huebner; C M Croce
Journal:  J Cell Physiol       Date:  1979-03       Impact factor: 6.384

9.  Kinetics of accumulation and processing of simian virus 40 RNA in Xenopus laevis oocytes injected with simian virus 40 DNA.

Authors:  T J Miller; D L Stephens; J E Mertz
Journal:  Mol Cell Biol       Date:  1982-12       Impact factor: 4.272

10.  Synthesis of ribosomal RNA in synkaryons and heterokaryons formed between human and rodent cells.

Authors:  C J Marshall
Journal:  J Cell Sci       Date:  1975-03       Impact factor: 5.285

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  8 in total

1.  Effects of single-base substitutions within the Acanthamoeba castellanii rRNA promoter on transcription and on binding of transcription initiation factor and RNA polymerase I.

Authors:  P Kownin; E Bateman; M R Paule
Journal:  Mol Cell Biol       Date:  1988-02       Impact factor: 4.272

2.  The ribosomal RNA promoter of Acanthamoeba castellanii determined by transcription in a cell-free system.

Authors:  P Kownin; C T Iida; S Brown-Shimer; M R Paule
Journal:  Nucleic Acids Res       Date:  1985-09-11       Impact factor: 16.971

Review 3.  Transcription of eukaryotic ribosomal RNA gene.

Authors:  S T Jacob
Journal:  Mol Cell Biochem       Date:  1986-04       Impact factor: 3.396

4.  DNA sequences for typical ribosomal gene spacers from Xenopus laevis and Xenopus borealis.

Authors:  P Labhart; R H Reeder
Journal:  Nucleic Acids Res       Date:  1987-04-24       Impact factor: 16.971

5.  In vitro mutagenesis and transcriptional analysis of a mouse ribosomal promoter element.

Authors:  J A Skinner; A Ohrlein; I Grummt
Journal:  Proc Natl Acad Sci U S A       Date:  1984-04       Impact factor: 11.205

6.  DNase I footprinting shows three protected regions in the promoter of the rRNA genes of Xenopus laevis.

Authors:  M Dunaway; R H Reeder
Journal:  Mol Cell Biol       Date:  1985-02       Impact factor: 4.272

7.  An RNA polymerase I promoter located in the CHO and mouse ribosomal DNA spacers: functional analysis and factor and sequence requirements.

Authors:  J Tower; S L Henderson; K M Dougherty; P J Wejksnora; B Sollner-Webb
Journal:  Mol Cell Biol       Date:  1989-04       Impact factor: 4.272

8.  Localization of the in vivo and in vitro transcription initiation site and comparative analysis of the flanking sequences in the two main size classes of Ascaris lumbricoides rDNA.

Authors:  G Briner; E Müller; H Neuhaus; E Back; F Müller; H Tobler
Journal:  Nucleic Acids Res       Date:  1987-08-25       Impact factor: 16.971

  8 in total

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