Literature DB >> 6323512

A simple procedure for the analysis of the structural proteins of influenza and parainfluenza viruses involving adsorption to erythrocytes.

J A Cowley, G A Tannock, R D Barry.   

Abstract

A simple procedure for the analysis of the structural proteins of influenza and parainfluenza viruses utilizing adsorption to erythrocytes is described. The method involves virus growth in the presence of [35S]methionine, adsorption of clarified culture medium with a 0.5% suspension of either guinea-pig or chicken erythrocytes and analysis of the virus-erythrocyte aggregates by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). All of the structural proteins can be detected using this procedure, and the protein profiles of virus-adsorbed erythrocyte complexes compare extremely well with those of sucrose density gradient purified virus preparations.

Entities:  

Mesh：

Substances：

Year:  1984        PMID： 6323512      PMCID： PMC7173268          DOI： 10.1016/0166-0934(84)90036-3

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  17 in total

1.  Influenza virus nucleic acid: relationship between biological characteristics of the virus particle and properties of the nucleic acid.

Authors:  G L ADA; B T PERRY
Journal:  J Gen Microbiol       Date:  1956-07

2.  Synthesis of influenza virus proteins in infected cells: translation of viral polypeptides, including three P polypeptides, from RNA produced by primary transcription.

Authors:  R A Lamb; P W Choppin
Journal:  Virology       Date:  1976-10-15       Impact factor: 3.616

3.  Inhibition of glycoprotein biosynthesis of influenza virus by D-glucosamine and 2-deoxy-D-glucose.

Authors:  H D Klenk; C Scholtissek; R Rott
Journal:  Virology       Date:  1972-09       Impact factor: 3.616

4.  The polypeptides of influenza virus. V. Localization of polypeptides in the virion by iodination techniques.

Authors:  P Stanley; E A Haslam
Journal:  Virology       Date:  1971-12       Impact factor: 3.616

5.  Influenza virus structural and nonstructural proteins in infected cells and their plasma membranes.

Authors:  S G Lazarowitz; R W Compans; P W Choppin
Journal:  Virology       Date:  1971-12       Impact factor: 3.616

6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

7.  A comparison of paramyxoviruses by immunoprecipitation.

Authors:  K K Goswami; W C Russell
Journal:  J Gen Virol       Date:  1982-05       Impact factor: 3.891

8.  High yield growth and purification of human parainfluenza type 3 virus and initial analysis of viral structural proteins.

Authors:  L E Guskey; G Bergtrom
Journal:  J Gen Virol       Date:  1981-05       Impact factor: 3.891

9.  Polypeptide synthesis in influenza virus-infected cells.

Authors:  J J Skehel
Journal:  Virology       Date:  1972-07       Impact factor: 3.616

10.  Characterization of human parainfluenza viruses. I. The structural proteins of parainfluenza virus 2 and their synthesis in infected cells.

Authors:  J A Cowley; R D Barry
Journal:  J Gen Virol       Date:  1983-10       Impact factor: 3.891
View more
  2 in total

1.  Genetic variants of influenza A/Taiwan/1/86 cocirculating in Canada during the winter of 1986 to 1987.

Authors:  E G Brown
Journal:  J Clin Microbiol       Date:  1988-02       Impact factor: 5.948

2.  Persistent infections with Sendai virus and Newcastle disease viruses.

Authors:  P Lawton; Z Karimi; L Mancinelli; J T Seto
Journal:  Arch Virol       Date:  1986       Impact factor: 2.574
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.