Literature DB >> 6323271

Construction of a vector for cloning promoters in Bacillus subtilis.

L Band, D G Yansura, D J Henner.   

Abstract

A versatile vector for cloning DNA fragments containing promoter activity in Bacillus subtilis was derived from plasmids pBR322, pUB110 and pC194. Selection is based on chloramphenicol resistance which is dependent upon the introduction of DNA fragments allowing expression of a chloramphenicol acetyl transferase gene. The plasmid contains a second selectable marker, neomycin resistance, and contains functional origins of replication for both B. subtilis and Escherichia coli.

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Year:  1983        PMID： 6323271     DOI： 10.1016/0378-1119(83)90204-4

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  17 in total

1.  Cloning and characterization of the str operon and elongation factor Tu expression in Bacillus stearothermophilus.

Authors:  L Krásný; T Vacík; V Fucík; J Jonák
Journal:  J Bacteriol       Date:  2000-11       Impact factor: 3.490

2.  Amy as a reporter gene for promoter activity in Nocardia lactamdurans: comparison of promoters of the cephamycin cluster.

Authors:  V K Chary; J L de la Fuente; P Liras; J F Martin
Journal:  Appl Environ Microbiol       Date:  1997-08       Impact factor: 4.792

3.  Nucleotide sequence of the kanamycin resistance determinant of the pneumococcal transposon Tn1545: evolutionary relationships and transcriptional analysis of aphA-3 genes.

Authors:  F Caillaud; P Trieu-Cuot; C Carlier; P Courvalin
Journal:  Mol Gen Genet       Date:  1987-05

4.  Promoters of the phr gene in Escherichia coli K-12.

Authors:  C Ma; C S Rupert
Journal:  Mol Gen Genet       Date:  1995-07-22

5.  Mutations that relieve nutritional repression of the Bacillus subtilis dipeptide permease operon.

Authors:  F J Slack; J P Mueller; A L Sonenshein
Journal:  J Bacteriol       Date:  1993-08       Impact factor: 3.490

6.  Expression of divIB of Bacillus subtilis during vegetative growth.

Authors:  E J Harry; S L Rowland; M S Malo; R G Wake
Journal:  J Bacteriol       Date:  1994-02       Impact factor: 3.490

7.  Cloning and analysis of the spc ribosomal protein operon of Bacillus subtilis: comparison with the spc operon of Escherichia coli.

Authors:  T M Henkin; S H Moon; L C Mattheakis; M Nomura
Journal:  Nucleic Acids Res       Date:  1989-09-25       Impact factor: 16.971

8.  Transcription of genes involved in the earliest steps of actinorhodin biosynthesis in Streptomyces coelicolor.

Authors:  V Parro; D A Hopwood; F Malpartida; R P Mellado
Journal:  Nucleic Acids Res       Date:  1991-05-25       Impact factor: 16.971

9.  Regulation of expression of Streptococcus mutans genes important to virulence.

Authors:  M C Hudson; R Curtiss
Journal:  Infect Immun       Date:  1990-02       Impact factor: 3.441

10.  Construction of a promoter-probe vector for a Bacillus subtilis host by using the trpD+ gene of Bacillus amyloliquefaciens.

Authors:  K Yoshimura; J Uemura; T Seki; Y Oshima
Journal:  J Bacteriol       Date:  1984-09       Impact factor: 3.490
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