Literature DB >> 6322300

Total synthesis and cloning of a gene coding for the ribonuclease S protein.

K P Nambiar, J Stackhouse, D M Stauffer, W P Kennedy, J K Eldredge, S A Benner.   

Abstract

A gene for ribonuclease S protein, has been chemically synthesized and cloned. The gene is designed to have 25 specific restriction endonuclease sites spaced at short intervals, permitting its structure to be rapidly modified. This flexibility facilitates tests of hypotheses relating the primary structure of the enzyme to its physical and catalytic behavior.

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Year:  1984        PMID: 6322300     DOI: 10.1126/science.6322300

Source DB:  PubMed          Journal:  Science        ISSN: 0036-8075            Impact factor:   47.728


  13 in total

1.  Life after the synthetic cell.

Authors:  Mark Bedau; George Church; Steen Rasmussen; Arthur Caplan; Steven Benner; Martin Fussenegger; Jim Collins; David Deamer
Journal:  Nature       Date:  2010-05-27       Impact factor: 49.962

2.  Simple and accurate estimation of ancestral protein sequences.

Authors:  Barry G Hall
Journal:  Proc Natl Acad Sci U S A       Date:  2006-03-27       Impact factor: 11.205

3.  A comprehensive list of chemically synthesized genes.

Authors:  G Gröger; F Ramalho-Ortigao; H Steil; H Seliger
Journal:  Nucleic Acids Res       Date:  1988-08-25       Impact factor: 16.971

4.  Computer management of oligonucleotide synthesis on cellulose filters.

Authors:  C M Tolstoshev; H W Matthes; P Oudet
Journal:  Nucleic Acids Res       Date:  1986-01-10       Impact factor: 16.971

Review 5.  Site-directed mutagenesis.

Authors:  P Carter
Journal:  Biochem J       Date:  1986-07-01       Impact factor: 3.857

6.  Codon-modifications and an endoplasmic reticulum-targeting sequence additively enhance expression of an Aspergillus phytase gene in transgenic canola.

Authors:  Ri-He Peng; Quan-Hong Yao; Ai-Sheng Xiong; Zong-Ming Cheng; Yi Li
Journal:  Plant Cell Rep       Date:  2005-10-25       Impact factor: 4.570

7.  Codon preference optimization increases heterologous PEDF expression.

Authors:  Anzor G Gvritishvili; Kar Wah Leung; Joyce Tombran-Tink
Journal:  PLoS One       Date:  2010-11-30       Impact factor: 3.240

8.  GeneDesign: rapid, automated design of multikilobase synthetic genes.

Authors:  Sarah M Richardson; Sarah J Wheelan; Robert M Yarrington; Jef D Boeke
Journal:  Genome Res       Date:  2006-02-15       Impact factor: 9.043

9.  The design of synthetic genes.

Authors:  S R Presnell; S A Benner
Journal:  Nucleic Acids Res       Date:  1988-03-11       Impact factor: 16.971

10.  Engineering proteinase K using machine learning and synthetic genes.

Authors:  Jun Liao; Manfred K Warmuth; Sridhar Govindarajan; Jon E Ness; Rebecca P Wang; Claes Gustafsson; Jeremy Minshull
Journal:  BMC Biotechnol       Date:  2007-03-26       Impact factor: 2.563

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