Literature DB >> 6322239

The separation of leukotrienes and hydroxyeicosatetraenoic acid metabolites of arachidonic acid by high performance liquid chromatography (HPLC).

D J Osborne, B J Peters, C J Meade.   

Abstract

The following high performance liquid chromatography system was found suitable for separating most lipoxygenase metabolites of arachidonic acid: Techsphere 5-C18 column, eluting solvent methanol:water:acetic acid (65:35:0.06 v/v), pH 5.3. Comparisons with other packing materials and solvent systems are described. The method could be used to identify lipoxygenase products released from mouse macrophage cells stimulated with gamma-hexachlorocyclohexane. Detection limits between 1 and 10 ng were obtained.

Entities:  

Mesh：

Substances：

Year:  1983        PMID： 6322239     DOI： 10.1016/0090-6980(83)90065-5

Source DB:  PubMed          Journal:  Prostaglandins        ISSN： 0090-6980


  6 in total

1.  Reduction of lipoxygenase products in psoriatic skin homogenates by QA 208-199.

Authors:  J Schnyder; T Hunziker; M Strasser; B Richardson; A Krebs
Journal:  Arch Dermatol Res       Date:  1986       Impact factor: 3.017

2.  The role of polyphosphoinositides and their breakdown products in A23187-induced release of arachidonic acid from rabbit polymorphonuclear leucocytes.

Authors:  C J Meade; G A Turner; P E Bateman
Journal:  Biochem J       Date:  1986-09-01       Impact factor: 3.857

3.  Arachidonic acid products and the thoracic accumulation of neutrophils and platelets following i.v. antigen challenge of sensitised guinea-pig.

Authors:  J R Walker; S A Woodhouse
Journal:  Agents Actions       Date:  1989-01

4.  Oxidative transformations of arachidonic acid in human dispersed lung cells: disparity between the utilization of endogenous and exogenous substrate.

Authors:  J Harvey; S T Holgate; B J Peters; C Robinson; J R Walker
Journal:  Br J Pharmacol       Date:  1985-10       Impact factor: 8.739

5.  New Class of 5-Lipoxygenase Inhibitors: Correlation Between Inhibition of LTB4 Production and Chemiluminescence of Human Polymorphonuclear Granulocytes.

Authors:  D Steinhilber; K Schmidt; K Eger; H J Roth
Journal:  Pharm Res       Date:  1986-10       Impact factor: 4.200

6.  Leukotrienes in the sputum and urine of cystic fibrosis children.

Authors:  A P Sampson; D A Spencer; C P Green; P J Piper; J F Price
Journal:  Br J Clin Pharmacol       Date:  1990-12       Impact factor: 4.335
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.