Literature DB >> 6321234

Nucleotide sequence of a yeast tRNAArg3A gene and its transcription in a homologous in vitro system.

J Villanueva, P Bull, P Valenzuela, A Venegas.   

Abstract

Twelve bacterial clones containing complementary sequences to yeast tRNAArg3 were isolated from a gene library. The size of the yeast BamHI inserts ranges from 5.4 to 10 MDa. There are at least 6 copies of this gene in different loci of the yeast genome. Insert from clone pYAT-3 was mapped, and the presence of a tRNAArg3A gene was confirmed by DNA sequence. The coding region is colinear with the transcriptional product. Unlike other reported tRNAArg3A genes, this one is not linked to a tRNAAsp gene. In vitro transcription using a yeast extract produces a transcript of 76 +/- 1 bases.

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Year:  1984        PMID： 6321234     DOI： 10.1016/0014-5793(84)80854-6

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  4 in total

1.  The mammalian upstream element factor recognizes two sites in the adenovirus type 2 IVa2-major late promoter intergenic region and stimulates both promoters.

Authors:  V Moncollin; A Kempf; J M Egly
Journal:  J Virol       Date:  1990-07       Impact factor: 5.103

2.  Genomic footprinting of a yeast tRNA gene reveals stable complexes over the 5'-flanking region.

Authors:  J M Huibregtse; D R Engelke
Journal:  Mol Cell Biol       Date:  1989-08       Impact factor: 4.272

3.  Isolation of a Saccharomyces cerevisiae centromere DNA-binding protein, its human homolog, and its possible role as a transcription factor.

Authors:  R J Bram; R D Kornberg
Journal:  Mol Cell Biol       Date:  1987-01       Impact factor: 4.272

4.  A library of yeast genomic MCM1 binding sites contains genes involved in cell cycle control, cell wall and membrane structure, and metabolism.

Authors:  M H Kuo; E Grayhack
Journal:  Mol Cell Biol       Date:  1994-01       Impact factor: 4.272
  4 in total

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