Effect of vitamins, antioxidants and sulfhydryl compounds on in vitro rat brain lipid peroxidation.

The effect of retinol, retinylacetate, alpha-tocopherol, alpha-tocopherylacetate, synthetic antioxidants (DPPD, BHT, DAH, and Ethoxyquine) sulfhydryl compounds (reduced glutathione and cysteine) on rat brain lipid peroxidation has been studied using the TBA method. Retinol and retinyl acetate inhibit brain lipid peroxidation, alpha-tocopherol is less effective than vitamin A, while alpha-tocopherol acetate has no antioxidant property. Ascorbic acid stimulates the peroxidation, at pH 5.0. The synthetic antioxidants are all potent inhibitors of brain lipid peroxidation, DPPD being the most effective, while the sulfhydryl compounds at a concentration of 1 x 10(-3)M has a slight potentiating effect.