Literature DB >> 6319395

Identification of Saccharomyces cerevisiae mutants deficient in DNA topoisomerase I activity.

C Thrash, K Voelkel, S DiNardo, R Sternglanz.   

Abstract

Mutants of the yeast, Saccharomyces cerevisiae, deficient in DNA topoisomerase I activity have been identified. One mutant has normal topoisomerase I activity when assayed at 25 degrees C and about 20% of normal activity when assayed at 36 degrees C. Strains with this mutation grow normally at all temperatures tested. The mutation has been mapped to MAK1, a gene required for maintenance of killer RNA. Three previously isolated mak1 mutants exhibit less than 1% of normal topoisomerase I activity in our assay, but yet they grow normally. The implications of these results for the role of DNA topoisomerase I in the cell are discussed.

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Year:  1984        PMID： 6319395

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  42 in total

Review 1.  Yeast killer systems.

Authors:  W Magliani; S Conti; M Gerloni; D Bertolotti; L Polonelli
Journal:  Clin Microbiol Rev       Date:  1997-07       Impact factor: 26.132

2.  Cloning and sequencing of Schizosaccharomyces pombe DNA topoisomerase I gene, and effect of gene disruption.

Authors:  T Uemura; K Morino; S Uzawa; K Shiozaki; M Yanagida
Journal:  Nucleic Acids Res       Date:  1987-12-10       Impact factor: 16.971

3.  Expression of yeast DNA topoisomerase I can complement a conditional-lethal DNA topoisomerase I mutation in Escherichia coli.

Authors:  M A Bjornsti; J C Wang
Journal:  Proc Natl Acad Sci U S A       Date:  1987-12       Impact factor: 11.205

4.  Topoisomerase I-mediated integration of hepadnavirus DNA in vitro.

Authors:  H P Wang; C E Rogler
Journal:  J Virol       Date:  1991-05       Impact factor: 5.103

5.  Inactivation of topoisomerase I or II may lead to recombination or to aberrant replication termination on both SV40 and yeast 2 micron DNA.

Authors:  P Levac; T Moss
Journal:  Chromosoma       Date:  1996-10       Impact factor: 4.316

Review 6.  Double-stranded RNA viruses of Saccharomyces cerevisiae.

Authors:  R B Wickner
Journal:  Microbiol Rev       Date:  1996-03

7.  Interaction between the N-terminus of human topoisomerase I and SV40 large T antigen.

Authors:  P Haluska; A Saleem; T K Edwards; E H Rubin
Journal:  Nucleic Acids Res       Date:  1998-04-01       Impact factor: 16.971

8.  Evidence for a nucleotide-dependent topoisomerase activity from yeast mitochondria.

Authors:  U R Ezekiel; E M Towler; J W Wallis; H P Zassenhaus
Journal:  Curr Genet       Date:  1994-12       Impact factor: 3.886

9.  Translation and M1 double-stranded RNA propagation: MAK18 = RPL41B and cycloheximide curing.

Authors:  K Carroll; R B Wickner
Journal:  J Bacteriol       Date:  1995-05       Impact factor: 3.490

10.  Isolation of mutants of Saccharomyces cerevisiae requiring DNA topoisomerase I.

Authors:  B U Sadoff; S Heath-Pagliuso; I B Castaño; Y Zhu; F S Kieff; M F Christman
Journal:  Genetics       Date:  1995-10       Impact factor: 4.562
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