Literature DB >> 6319192

Expression of human alpha 1-antitrypsin in Escherichia coli.

A Bollen, R Loriau, A Herzog, P Hérion.   

Abstract

Complementary DNA coding for human alpha 1-antitrypsin has been placed under the control of the lambda PR promotor carrier by the expression vector pCQV2 [1]. In conditions which allow transcription from this promotor (thermoinactivation of the repressor), Escherichia coli cells harbouring the recombinant plasmid pULB1114 express human alpha 1-antitrypsin (+/- 9000 molecules/cell). The product has a Mr of 44000, corresponding to mature unglycosylated alpha 1-antitrypsin.

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Year:  1984        PMID： 6319192     DOI： 10.1016/0014-5793(84)80046-0

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  5 in total

1.  Expression of the cloned bacteriophage phi X174 A* gene in Escherichia coli inhibits DNA replication and cell division.

Authors:  J Colasanti; D T Denhardt
Journal:  J Virol       Date:  1985-03       Impact factor: 5.103

2.  Molecular cloning and expression of an intracellular serpin: an elastase inhibitor from horse leucocytes.

Authors:  T Kordula; A Dubin; H Schooltink; A Koj; P C Heinrich; S Rose-John
Journal:  Biochem J       Date:  1993-07-01       Impact factor: 3.857

3.  Neonatal hepatitis with obstructive jaundice in an SZ heterozygous alpha 1-antitrypsin-deficient boy and destructive lung disease in his SZ mother. A review of the literature.

Authors:  Y Vandenplas; J Franckx; I Liebaers; P Ketelbant; L Sacre
Journal:  Eur J Pediatr       Date:  1985-11       Impact factor: 3.183

4.  Expression of the alpha-1-antitrypsin gene in mononuclear phagocytes of normal and alpha-1-antitrypsin-deficient individuals.

Authors:  J F Mornex; A Chytil-Weir; Y Martinet; M Courtney; J P LeCocq; R G Crystal
Journal:  J Clin Invest       Date:  1986-06       Impact factor: 14.808

5.  Expression of human alpha 1-antitrypsin cDNA in the yeast Saccharomyces cerevisiae.

Authors:  T Cabezón; M De Wilde; P Herion; R Loriau; A Bollen
Journal:  Proc Natl Acad Sci U S A       Date:  1984-11       Impact factor: 11.205
  5 in total

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