Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Concentration of rotavirus and enteroviruses from blue crabs (Callinectes sapidus).

Literature DB >> 6318667

Concentration of rotavirus and enteroviruses from blue crabs (Callinectes sapidus).

K M Seidel, S M Goyal, V C Rao, J L Melnick.

Abstract

A simple method for concentration and detection of rotavirus and enteroviruses in the blue crab is described. Virus was separated from tissue homogenates at pH 9.5, concentrated by adsorption to protein precipitates at pH 3.5, and recovered by elution of precipitates at pH 9.2. Test samples of 12 to 15 ml were produced from an initial 100 g of crab tissues. Cat-floc precipitation was used to remove sample toxicity for cell cultures. Recovery effectiveness averaged 52% with poliovirus 1, echovirus 7, and coxsackievirus B5 and 23% with rotavirus SA11.

Entities: Disease Species

Mesh：

Year: 1983 PMID： 6318667 PMCID： PMC239566 DOI： 10.1128/aem.46.6.1293-1296.1983

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

13 in total

Concentration of rotavirus and enteroviruses from blue crabs (Callinectes sapidus).

1. A plaque assay for the simian rotavirus SAII.

2. Human enteroviruses in oysters and their overlying waters.

3. Development of a simple method for concentrating enteroviruses from oysters.

4. Improved methods for detecting enteric viruses in oysters.

5. Polyelectrolyte flocculation as an aid to recovery of enteroviruses from oysters.

6. Preliminary observations on the uptake poliovirus by West Coast shore crabs.

7. Uptake of bacteriophage and their subsequent survival in edible West Coast crabs after processing.

8. Rotavirus stability and inactivation.

9. Aggregation of poliovirus and reovirus by dilution in water.

10. Survival of virus in chilled, frozen, and processed oysters.

1. Methods for recovering poliovirus and rotavirus from oysters.

2. Early Days of Food and Environmental Virology.

3. Human pathogenic viruses at sewage sludge disposal sites in the Middle Atlantic region.

Review 4. Pathogenic human viruses in coastal waters.

Review 5. Innate antiviral defenses in body fluids and tissues.