Literature DB >> 631864

Occurrence of 1st division metaphases in human lymphocyte cultures.

P E Crossen, W F Morgan.   

Abstract

Human lymphocytes were cultured for 40, 42 and 44 h and analysed for the number of 1st and 2nd division metaphases using the Brd U-Giemsa technique. The most suitable time to obtain the majority of cells in their 1st division concommittant with an adequate mitotic index was 42 h. It is recommended that laboratories use sister chromatid differential staining to determine the most suitable culture time for the analysis of 1st division metaphases. This will permit accurate comparisons between individuals and laboratories to be made.

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Year:  1978        PMID： 631864     DOI： 10.1007/bf00278875

Source DB:  PubMed          Journal:  Hum Genet        ISSN： 0340-6717            Impact factor:   4.132


  10 in total

1.  The human leukocyte test system. V. DNA synthesis and mitoses in PHA-stimulated 3-day cultures.

Authors:  G Obe; B Beek; G Dudin
Journal:  Humangenetik       Date:  1975-08-25

2.  Human peripheral blood lymphocytes for the analysis of chromosome aberrations in mutagen tests.

Authors:  H J Evans; M L O'Riordan
Journal:  Mutat Res       Date:  1975-06       Impact factor: 2.433

3.  Chromosome aberrations in lymphocytes after occupational exposure to lead and cadmium.

Authors:  M Bauchinger; E Schmid; H J Einbrodt; J Dresp
Journal:  Mutat Res       Date:  1976-01       Impact factor: 2.433

Review 4.  Induced chromosomal aberrations in man.

Authors:  A D Bloom
Journal:  Adv Hum Genet       Date:  1972

5.  Sister chromatid exchanges induced by light flashes to 5-bromodeoxyuridine- and 5-iododeoxyuridine substituted Chinese hamster chromosomes.

Authors:  T Ikushima; S Wolff
Journal:  Exp Cell Res       Date:  1974-07       Impact factor: 3.905

6.  Giemsa banding patterns of human chromosomes.

Authors:  P E Crossen
Journal:  Clin Genet       Date:  1972       Impact factor: 4.438

7.  Analysis of human lymphocyte cell cycle time in culture measured by sister chromatid differential staining.

Authors:  P E Crossen; W F Morgan
Journal:  Exp Cell Res       Date:  1977-02       Impact factor: 3.905

8.  Giemsa technique for the detection of sister chromatid exchanges.

Authors:  J R Korenberg; E F Freedlender
Journal:  Chromosoma       Date:  1974       Impact factor: 4.316

9.  Cytogenetic effects of inhaled ozone in man.

Authors:  W H McKenzie; J H Knelson; N J Rummo; D E House
Journal:  Mutat Res       Date:  1977       Impact factor: 2.433

10.  Chromosomal radiosensitivity and karyotype in mice using cultured peripheral blood lymphocytes, and comparison with this system in man.

Authors:  P De Boer; P P Van Buul; R Van Beek; F A Van Der Hoeven; T Natarajan
Journal:  Mutat Res       Date:  1977-03       Impact factor: 2.433
  10 in total
  3 in total

1.  Sister chromatid exchange in cigarette smokers.

Authors:  P E Crossen; W F Morgan
Journal:  Hum Genet       Date:  1980       Impact factor: 4.132

2.  Frequency and types of induced and spontaneous chromosome aberrations in relation to cell kinetics.

Authors:  O Mutchinick; L Ruz; M E Gonsebatt; P Mauleón; R Lisker; G García
Journal:  Hum Genet       Date:  1981       Impact factor: 4.132

3.  Assessment of Potential In vitro Genotoxic and Cytotoxic Effects of Bupropion Hydrochloride (Wellbutrin) in Human Peripheral Lymphocytes and Human Cortical Neuron.

Authors:  Saurabh Kumar Bhattacharya; Lokendra Singh Nathawat; Priyankka Damani; Arpan Kumar Choksi; Arpita Banik; Kriti Sinha; Aditi Sarkar Bhattacharya
Journal:  Toxicol Int       Date:  2013-01
  3 in total

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