| Literature DB >> 6317755 |
S V Hetherington, J K Spitznagel, P G Quie.
Abstract
An enzyme-linked immunosorbent assay has been developed for quantitation of lactoferrin (LF) in body fluids. An indirect double-sandwich method was used which allows a sensitivity of 3 ng LF/ml in samples of polymorphonuclear cell lysates and serum. Mean LF content of serum was 0.307 +/- 0.066 micrograms/ml (n = 18). Mean LF content of polymorphonuclear cells was 4.90 +/- 1.48 micrograms/10(6) PMN. Concentrations of LF were similar in serum and in plasma of EDTA anticoagulated blood. Advantages of this method include its rapidity, and radioactivity is not required.Entities:
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Year: 1983 PMID： 6317755 DOI： 10.1016/0022-1759(83)90314-9
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303