Hormonal sensitivity of brown fat cells of fetal rats in monolayer culture.

The primary monolayer culture of brown fat cells was performed with the brown adipose tissue of fetal rats as the material. Brown fat cells in the culture were highly sensitive to some hormones. Insulin had a marked effect of lipogenesis and it made the cells contain large multilocular lipid droplets. Lipolysis is also mediated by hormones, e.g., epinephrine or ACTH etc., and lipolytic process was controlled through cyclic AMP system. Brown fat cells of fetal rats in monolayer culture seem to be a suitable material for long-term experiment of lipogenesis and lipolysis.