Literature DB >> 6316854

Simple screening method for isolation of penicillin acylase-producing bacteria.

V Meevootisom, P Somsuk, R Prachaktam, T W Flegel.   

Abstract

A new screening method for bacteria capable of producing penicillin acylase is described. The method is based on the use of Serratia marcescens sensitive to 6-aminopenicillanic acid but comparatively resistant to benzylpenicillin. It is simple, quite specific, and requires no special equipment. It can also be used to screen for phenoxymethylpenicillin acylase activity. We also suggest an acidimetric method for rapid detection of cloned genes in genetic engineering studies of penicillin acylase.

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Year:  1983        PMID： 6316854      PMCID： PMC239545          DOI： 10.1128/aem.46.5.1227-1229.1983

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  7 in total

1.  Susceptibility of Haemophilus influenza to ampicillin as determined by use of a modified, one-minute beta-lactamase test.

Authors:  J Escamilla
Journal:  Antimicrob Agents Chemother       Date:  1976-01       Impact factor: 5.191

2.  A method for isolating bacteria capable of producing 6-aminopenicillanic acid from benzylpenillin.

Authors:  Y KAMEDA; Y KIMURA; E TOYOURA; T OMORI
Journal:  Nature       Date:  1961-09-09       Impact factor: 49.962

3.  The isolation and kinetics of penicillin amidase from Escherichia coli.

Authors:  K Balasingham; D Warburton; P Dunnill; M D Lilly
Journal:  Biochim Biophys Acta       Date:  1972-07-13

4.  A quantitative microbiological determination of 6-aminopenicillanic acid.

Authors:  J G Oostendorp
Journal:  Antonie Van Leeuwenhoek       Date:  1972       Impact factor: 2.271

5.  A note on the detection of penicillin acylase activity in Escherichia coli by the reaction of ampicillin with Biuret reagent.

Authors:  W L Baker
Journal:  J Appl Bacteriol       Date:  1980-10

6.  Colorimetric assay of penicillin amidase activity using phenylacetyl-aminobenzoic acid as substrate.

Authors:  A Szewczuk; M Siewiński; R Słowińska
Journal:  Anal Biochem       Date:  1980-03-15       Impact factor: 3.365

7.  Iodometric detection of Haemophilus influenzae beta-lactamase: rapid presumptive test for ampicillin resistance.

Authors:  B W Catlin
Journal:  Antimicrob Agents Chemother       Date:  1975-03       Impact factor: 5.191
  7 in total
  5 in total

1.  Chaperone-mediated folding and maturation of the penicillin acylase precursor in the cytoplasm of Escherichia coli.

Authors:  Yali Xu; Chiao-Ling Weng; Niju Narayanan; Ming-Yi Hsieh; William A Anderson; Jeno M Scharer; Murray Moo-Young; C Perry Chou
Journal:  Appl Environ Microbiol       Date:  2005-10       Impact factor: 4.792

2.  Developing an extended genomic engineering approach based on recombineering to knock-in heterologous genes to Escherichia coli genome.

Authors:  Karan Sukhija; Michael Pyne; Saad Ali; Valerie Orr; Daryoush Abedi; Murray Moo-Young; C Perry Chou
Journal:  Mol Biotechnol       Date:  2012-06       Impact factor: 2.695

3.  Expression of the Arthrobacter viscosus penicillin G acylase gene in Escherichia coli and Bacillus subtilis.

Authors:  H Ohashi; Y Katsuta; M Nagashima; T Kamei; M Yano
Journal:  Appl Environ Microbiol       Date:  1989-06       Impact factor: 4.792

4.  Molecular cloning of the penicillin G acylase gene from Arthrobacter viscosus.

Authors:  H Ohashi; Y Katsuta; T Hashizume; S N Abe; H Kajiura; H Hattori; T Kamei; M Yano
Journal:  Appl Environ Microbiol       Date:  1988-11       Impact factor: 4.792

5.  Protein engineering of penicillin acylase.

Authors:  V I Tishkov; S S Savin; A S Yasnaya
Journal:  Acta Naturae       Date:  2010-07       Impact factor: 1.845
  5 in total

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