Literature DB >> 6316644

The pattern of integration of viral DNA sequences in the adenovirus 5-transformed human cell line 293.

D J Spector.   

Abstract

The pattern of integrated adenovirus 5 DNA in the adenovirus 5-transformed human cell line 293 was analyzed by DNA blot hybridization. In contrast to a previous report, only sequences from the left end of the genome were detected. The viral DNA was contained in a unique DNA fragment generated by cleavage of 293 DNA with either EcoRI or BamHI, two enzymes which do not cut the integrated viral DNA. Further mapping studies indicated that the integrated sequence was probably colinear with viral DNA. The joining to host cell sequences occurred between viral nucleotide base pairs 1 and 270 (a site for BalI) on one end and 4123 (SmaI site) and 5372 (BalI site) at the other end. The viral DNA was not tandemly repeated. These results suggest that integration of viral sequences into the host genome probably occurred at a single site. If any subsequent duplications of viral DNA took place, then duplication of extensive cellular sequences also must have occurred.

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Year:  1983        PMID： 6316644     DOI： 10.1016/0042-6822(83)90107-1

Source DB:  PubMed          Journal:  Virology        ISSN： 0042-6822            Impact factor:   3.616


  11 in total

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Authors:  C L Parks; L S Chang; T Shenk
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2.  Standard free droplet digital polymerase chain reaction as a new tool for the quality control of high-capacity adenoviral vectors in small-scale preparations.

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3.  Adenovirus E1A gene autorepression: revertants of an E1A promoter mutation encode altered E1A proteins.

Authors:  P L Larsen; C Tibbetts
Journal:  Proc Natl Acad Sci U S A       Date:  1987-12       Impact factor: 11.205

4.  Two regions of the adenovirus early region 1A proteins are required for transformation.

Authors:  P Whyte; H E Ruley; E Harlow
Journal:  J Virol       Date:  1988-01       Impact factor: 5.103

5.  The E4 transcriptional unit of Ad2: far upstream sequences are required for its transactivation by E1A.

Authors:  P Gilardi; M Perricaudet
Journal:  Nucleic Acids Res       Date:  1984-10-25       Impact factor: 16.971

6.  Evaluation of cell line 293 for virus isolation in routine viral diagnosis.

Authors:  M Brown; M Petric
Journal:  J Clin Microbiol       Date:  1986-04       Impact factor: 5.948

7.  Identification of a region on the adenovirus E1A gene responsible for induction by phorbol ester tumor promoter.

Authors:  Q Gai; C B James
Journal:  In Vitro Cell Dev Biol Anim       Date:  2001 Jul-Aug       Impact factor: 2.416

8.  Molecular characterization of replication-competent variants of adenovirus vectors and genome modifications to prevent their occurrence.

Authors:  K M Hehir; D Armentano; L M Cardoza; T L Choquette; P B Berthelette; G A White; L A Couture; M B Everton; J Keegan; J M Martin; D A Pratt; M P Smith; A E Smith; S C Wadsworth
Journal:  J Virol       Date:  1996-12       Impact factor: 5.103

9.  Self-inactivating lentivirus vector for safe and efficient in vivo gene delivery.

Authors:  R Zufferey; T Dull; R J Mandel; A Bukovsky; D Quiroz; L Naldini; D Trono
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10.  In vitro and in vivo synthesis of the hepatitis B virus surface antigen and of the receptor for polymerized human serum albumin from recombinant human adenoviruses.

Authors:  A Ballay; M Levrero; M A Buendia; P Tiollais; M Perricaudet
Journal:  EMBO J       Date:  1985-12-30       Impact factor: 11.598
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