Literature DB >> 6315767

Evaluation of solubilized herpes simplex virus membrane antigen by enzyme-linked immunosorbent assay.

S Jeansson, M Forsgren, B Svennerholm.   

Abstract

An antigen prepared by solubilization of membranes from herpes simplex virus (HSV)-infected cells with deoxycholate was evaluated by enzyme-linked immunosorbent assay. The deoxycholate-solubilized antigen, previously shown to contain all major HSV glycoproteins, was noninfectious and adsorbed easily and reproducibly to a polystyrene surface at pH 9.6. The deoxycholate-solubilized antigen provided an enzyme-linked immunosorbent assay of high sensitivity and reproducibility with complete correlation with complement fixation for the diagnosis of acute HSV infection. The correlation with neutralization and immunofluorescence for the presence or absence of anti-HSV activity was very good. Comparison with an HSV envelope preparation yielded results slightly in favor of the deoxycholate-solubilized antigen. The assay seems to be useful for demonstration of intrathecal production of antibody activity in HSV encephalitis.

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Year:  1983        PMID： 6315767      PMCID： PMC272860          DOI： 10.1128/jcm.18.5.1160-1166.1983

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  17 in total

1.  Application of a microtechnique to viral serological investigations.

Authors:  J L SEVER
Journal:  J Immunol       Date:  1962-03       Impact factor: 5.422

2.  Preparation of type specific herpes simplex antisera by an immunosorbent method;.

Authors:  S Jeansson
Journal:  Acta Pathol Microbiol Scand B       Date:  1975-02

3.  Differentiation between herpes simplex virus type 1 and type 2 strains by immunoelectroosmophoresis.

Authors:  S Jeansson
Journal:  Appl Microbiol       Date:  1972-07

4.  Detection of cell-dependent cytotoxic antibody to cells infected with herpes simplex virus.

Authors:  S L Shore; A J Nahmias; S E Starr; P A Wood; D E McFarlin
Journal:  Nature       Date:  1974-09-27       Impact factor: 49.962

5.  Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.

Authors:  E Engvall; P Perlmann
Journal:  J Immunol       Date:  1972-07       Impact factor: 5.422

6.  Complement-fixing antigens of herpes simplex virus types 1 and 2: reactivity of capsid, envelope, and soluble antigens.

Authors:  M L Martin; E L Palmer; R E Kissling
Journal:  Infect Immun       Date:  1972-02       Impact factor: 3.441

7.  Immunological relationship between herpes simplex and varicella-zoster viruses demonstrated by complement-fixation, neutralization and fluorescent antibody tests.

Authors:  N J Schmidt; E H Lennette; R L Magoffin
Journal:  J Gen Virol       Date:  1969-04       Impact factor: 3.891

8.  Varicella virus in HeLa cells.

Authors:  A Svedmyr
Journal:  Arch Gesamte Virusforsch       Date:  1965

9.  Hemolysis-in-gel and neutralization tests for determination of antibodies to mumps virus.

Authors:  L Grillner; J Blomberg
Journal:  J Clin Microbiol       Date:  1976-07       Impact factor: 5.948

10.  Quantitation of antibodies to Herpes simplex virus types 1 and 2 by complement-dependent antibody lysis of infected cells.

Authors:  H McClung; P Seth; W E Rawls
Journal:  Am J Epidemiol       Date:  1976-08       Impact factor: 4.897
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  15 in total

1.  Interleukin-12 (IL-12) and IL-18 are important in innate defense against genital herpes simplex virus type 2 infection in mice but are not required for the development of acquired gamma interferon-mediated protective immunity.

Authors:  A M Harandi; B Svennerholm; J Holmgren; K Eriksson
Journal:  J Virol       Date:  2001-07       Impact factor: 5.103

2.  Acyclovir treatment of relapsing-remitting multiple sclerosis. A randomized, placebo-controlled, double-blind study.

Authors:  J Lycke; B Svennerholm; E Hjelmquist; L Frisén; G Badr; M Andersson; A Vahlne; O Andersen
Journal:  J Neurol       Date:  1996-03       Impact factor: 4.849

3.  Involvement of glycoprotein C (gC) in adsorption of herpes simplex virus type 1 (HSV-1) to the cell.

Authors:  B Svennerholm; S Jeansson; A Vahlne; E Lycke
Journal:  Arch Virol       Date:  1991       Impact factor: 2.574

4.  Cells producing antibody to measles and herpes simplex virus in cerebrospinal fluid and blood of patients with multiple sclerosis and controls.

Authors:  S Baig; O Olsson; T Olsson; A Löve; S Jeansson; H Link
Journal:  Clin Exp Immunol       Date:  1989-12       Impact factor: 4.330

5.  Prevalence of antibodies to herpes simplex virus types 1 and 2 in pregnant women, and estimated rates of infection.

Authors:  A E Ades; C S Peckham; G E Dale; J M Best; S Jeansson
Journal:  J Epidemiol Community Health       Date:  1989-03       Impact factor: 3.710

6.  Hamster model for herpes simplex virus infection of the central nervous system.

Authors:  L T van Ekdom; P Herbrink; M J Meddens
Journal:  Infection       Date:  1987 Mar-Apr       Impact factor: 3.553

7.  Long-term persistence of intrathecal virus-specific antibody responses after herpes simplex virus encephalitis.

Authors:  B Vandvik; B Sköldenberg; M Forsgren; G Stiernstedt; S Jeansson; E Norrby
Journal:  J Neurol       Date:  1985       Impact factor: 4.849

8.  Dichotomy of glycoprotein g gene in herpes simplex virus type 1 isolates.

Authors:  Elham Rekabdar; Petra Tunbäck; Jan-Ake Liljeqvist; Magnus Lindh; Tomas Bergström
Journal:  J Clin Microbiol       Date:  2002-09       Impact factor: 5.948

9.  Herpes simplex virus type 1-induced hemagglutination: glycoprotein C mediates virus binding to erythrocyte surface heparan sulfate.

Authors:  E Trybala; B Svennerholm; T Bergström; S Olofsson; S Jeansson; J L Goodman
Journal:  J Virol       Date:  1993-03       Impact factor: 5.103

10.  Secretory IgA against herpes simplex virus in cervical secretions.

Authors:  E Persson; P Eneroth; S Jeansson
Journal:  Genitourin Med       Date:  1988-12
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