Literature DB >> 6315750

A method for coupling cytomegalovirus antigens to aldehyde-fixed erythrocytes for use in passive hemagglutination.

H Ikram, A M Prince.   

Abstract

A method for coupling cytomegalovirus (CMV) antigens to aldehyde-fixed erythrocytes for use in a passive hemagglutination assay for antibody to CMV is described. This method uses acid buffer instead of tannic acid for coupling CMV-complement fixation (CF) antigen to the stabilized cells. The coated cells are stable for at least 6 mth at -70 degrees C and for 5 days at 4 degrees C. This provides an inexpensive reagent having a sensitivity for anti-CMV detection which compares favorably with that of the CF test and enzyme immunoassay.

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Year:  1983        PMID： 6315750     DOI： 10.1016/0166-0934(83)90002-2

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  3 in total

1.  Graft-versus-host disease is the major determinant of humoral responses to the AS03-adjuvanted influenza A/09/H1N1 vaccine in allogeneic hematopoietic stem cell transplant recipients.

Authors:  Bilal Mohty; Michael Bel; Marija Vukicevic; Monika Nagy; Emmanuel Levrat; Sara Meier; Stephane Grillet; Christophe Combescure; Laurent Kaiser; Yves Chalandon; Jakob Passweg; Claire-Anne Siegrist; Eddy Roosnek
Journal:  Haematologica       Date:  2011-03-21       Impact factor: 9.941

2.  Confirmation of human cytomegalovirus by reverse passive hemagglutination with monoclonal antibodies reactive to the major glycosylated peptide (GP-66).

Authors:  K S Kim; V Sapienza; C M Chen
Journal:  J Clin Microbiol       Date:  1986-09       Impact factor: 5.948

3.  A prospective study of the factors shaping antibody responses to the AS03-adjuvanted influenza A/H1N1 vaccine in cancer outpatients.

Authors:  Andreas F Hottinger; Anne-Claude C George; Michael Bel; Laurence Favet; Christophe Combescure; Sara Meier; Stéphane Grillet; Klara Posfay-Barbe; Laurent Kaiser; Claire-Anne Siegrist; Pierre-Yves Dietrich
Journal:  Oncologist       Date:  2012-02-21
  3 in total

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