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Literature DB >> 6315574

Mapping of class I DNA sequences within the human major histocompatibility complex.

Abstract

Mutants that had lost expression of alleles of one or more HLA loci were isolated with immunoselection after gamma-irradiation of a human lymphoblastoid cell line LCL 721. DNAs from the mutants were digested with restriction endonucleases and analyzed by Southern blotting using probes for class I HLA genes. Eight polymorphic cut sites for HindIII and PvuII were discovered in class I-associated sequences of LCL 721. Losses of specific fragments generated by restriction enzymes could be associated with losses of specific antigenic expressions and it was possible in this way to assign HLA-A1, HLA-A2, and HLA-B8 to specific DNA fragments. Patterns of gamma-ray-induced segregations of DNA fragments permitted rough linkage alignment of about 30% of the fragments generated by PvuII. The resultant map showed that there are class I HLA genes on the telomeric side of the HLA-A locus. Restriction enzyme site polymorphisms were also examined in a panel of DNAs isolated from peripheral blood lymphocytes (PBLs) of HLA-typed individuals. This panel of PBL DNA complemented the analysis using the HLA deletion mutants.

Entities: Gene Species

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Year: 1983 PMID： 6315574 DOI： 10.1007/bf00364390

Source DB: PubMed Journal: Immunogenetics ISSN： 0093-7711 Impact factor: 2.846

31 in total

1. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors: P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal: J Mol Biol Date: 1977-06-15 Impact factor: 5.469

2. Specificity and frequency of ultraviolet-induced reversion of an iso-1-cytochrome c ochre mutant in radiation-sensitive strains of yeast.

Authors: C W Lawrence; J W Stewart; F Sherman; R Christensen
Journal: J Mol Biol Date: 1974-05-05 Impact factor: 5.469

3. Isolation and partial nucleotide sequence of a cDNA clone for human histocompatibility antigen HLA-B by use of an oligodeoxynucleotide primer.

Authors: A K Sood; D Pereira; S M Weissman
Journal: Proc Natl Acad Sci U S A Date: 1981-01 Impact factor: 11.205

4. Expression of human class I histocompatibility antigens at the surface of DNA-transformed mouse L cells.

Authors: F A Lemonnier; M Malissen; P Golstein; P Le Bouteiller; N Rebai; M Damotte; D Birnbaum; D Caillol; J Trucy; B R Jordan
Journal: Immunogenetics Date: 1982 Impact factor: 2.846

5. cDNA clone coding for part of a mouse H-2d major histocompatibility antigen.

Authors: S Kvist; F Bregegere; L Rask; B Cami; H Garoff; F Daniel; K Wiman; D Larhammar; J P Abastado; G Gachelin; P A Peterson; B Dobberstein; P Kourilsky
Journal: Proc Natl Acad Sci U S A Date: 1981-05 Impact factor: 11.205

6. Dissection of the D-region of the human major histocompatibility complex by means of induced mutations in a lymphoblastoid cell line.

Authors: R DeMars; C C Chang; R A Rudersdorf
Journal: Hum Immunol Date: 1983-10 Impact factor: 2.850

Mapping of class I DNA sequences within the human major histocompatibility complex.

1. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

2. Specificity and frequency of ultraviolet-induced reversion of an iso-1-cytochrome c ochre mutant in radiation-sensitive strains of yeast.

3. Isolation and partial nucleotide sequence of a cDNA clone for human histocompatibility antigen HLA-B by use of an oligodeoxynucleotide primer.

4. Expression of human class I histocompatibility antigens at the surface of DNA-transformed mouse L cells.

5. cDNA clone coding for part of a mouse H-2d major histocompatibility antigen.

6. Dissection of the D-region of the human major histocompatibility complex by means of induced mutations in a lymphoblastoid cell line.

7. DNA sequence of a gene encoding a BALB/c mouse Ld transplantation antigen.

8. Molecular cloning of a human histocompatibility antigen cDNA fragment.

9. Three cDNA clones encoding mouse transplantation antigens: homology to immunoglobulin genes.

10. Class I-like HLA genes map telomeric to the HLA-A2 locus in human cells.

1. Nucleotide sequence of an HLA-A1 gene.

2. Nucleotide sequence of an HLA-Bw57 gene.

3. Isolation and characterization of yeast artificial chromosome clones linking the HLA-B and HLA-C loci.

4. A mutant rat major histocompatibility haplotype showing a large deletion of class I sequences.

Review 5. Organization of the human class I major histocompatibility complex genes.

6. A 3.5 kilobase Taq I restriction fragment of genomic DNA segregates with HLA-B27.

7. The HLA-AW24 gene: sequence, surroundings and comparison with the HLA-A2 and HLA-A3 genes.

8. Construction of a map of the short arm of human chromosome 6.

9. Transfer of cloned human class I major histocompatibility complex genes into HLA mutant human lymphoblastoid cells.

10. Mutations that impair a posttranscriptional step in expression of HLA-A and -B antigens.