Literature DB >> 6315550

Preparation of highly purified mitochondria of Neurospora crassa on a Percoll gradient.

H Fecíková, J Turna, Z Baráth.   

Abstract

Mitochondria isolated from Neurospora crassa were purified by centrifugation in a Percoll density gradient. Enzyme activities and cytochrome differential spectra revealed a high purity of the mitochondria. As compared with a crude mitochondrial fraction the purified mitochondria exhibited a high respiratory activity and a fine ADP/O ratio. Electrophoresis of nucleic acids demonstrated the absence of cytoplasmic rRNA.

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Year:  1983        PMID: 6315550     DOI: 10.1007/bf02879491

Source DB:  PubMed          Journal:  Folia Microbiol (Praha)        ISSN: 0015-5632            Impact factor:   2.099


  7 in total

1.  Spectrophotometric assay of cytochrome c oxidase.

Authors:  L SMITH
Journal:  Methods Biochem Anal       Date:  1955

2.  Oxidation processes and ubiquinone localization in the branched respiratory system of mi-1 mutant of Neurospora crassa.

Authors:  A K Drabikowska
Journal:  Acta Biochim Pol       Date:  1975       Impact factor: 2.149

3.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

4.  General methodology for isolation and characterization of mitochondria from microorganisms.

Authors:  D Lloyd
Journal:  Methods Enzymol       Date:  1979       Impact factor: 1.600

5.  Preparation and analysis of mitochondrial ribosomes.

Authors:  A M Lambowitz
Journal:  Methods Enzymol       Date:  1979       Impact factor: 1.600

6.  A method for quantitation of protein in the presence of Percoll.

Authors:  M N Khan; R J Khan; B I Posner
Journal:  Anal Biochem       Date:  1981-10       Impact factor: 3.365

7.  Cyanide-resistant respiration in Neurospora crassa.

Authors:  A M Lambowitz; C W Slayman
Journal:  J Bacteriol       Date:  1971-12       Impact factor: 3.490

  7 in total
  1 in total

1.  Hydrogen peroxide acts on sensitive mitochondrial proteins to induce death of a fungal pathogen revealed by proteomic analysis.

Authors:  Guozheng Qin; Jia Liu; Baohua Cao; Boqiang Li; Shiping Tian
Journal:  PLoS One       Date:  2011-07-06       Impact factor: 3.240

  1 in total

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