Electrophysiological study of paraventricular nucleus neurons projecting to the dorsomedial medulla and their response to baroreceptor stimulation in rats.

In male rats anesthetized with urethane, extracellular recordings were made from 415 neurons in the paraventricular nucleus (PVN) and adjacent areas. Of these neurons 64 were excited antidromically by stimulation of the dorsomedial medulla but not by stimulation of the pituitary stalk (first group). Seventy-three neurons were antidromically excited by stimulation of the pituitary stalk but not of the dorsomedial medulla (second group, neurosecretory cells). The other 2 neurons were antidromically excited by stimulation of both the dorsomedial medulla and the pituitary stalk (third group). Latencies of antidromically evoked action potentials by stimulation of the dorsomedial medulla and of the pituitary stalk ranged between 8 and 60 ms (mean +/- S.D., 38.5 +/- 9.8, n = 66) and from 7 to 24 ms (mean +/- S.D., 13.0 +/- 3.6, n = 75), respectively, suggesting unmyelinated fiber projections in both instances. PVN neurons of these 3 groups were found to be dispersed throughout the PVN and no difference in specific locations between the neuron groups existed. Their characteristics, however, were different. The first group of neurons discharged at a slower rate and showed no phasic pattern of firing, while 28% of the second group of neurons ('identified' neurosecretory cells) showed phasic patterns of firing and their rates of discharge were higher than those of the first group of neurons. The two neurons belonging to the third group showed irregular spontaneous discharges. The areas within the dorsomedial medulla stimulation of which evoked antidromic excitation of PVN neurons were located within and adjacent to the nucleus of the tractus solitarius (NTS) and the dorsal motor nucleus of the vagus (DMV). Among PVN neurons which were antidromically excited by stimulation of dorsomedial medulla, 51 cells were examined for their responses to excitation of baroreceptors. An increase in pressure of the 'isolated' carotid sinus excited 2 neurons, and inhibited 7 (14%). On the other hand, 27% (11 out of 41) of neurosecretory cells (second group) were inhibited by baroreceptor stimulation. From these results, it was concluded that essentially separate populations of PVN neurons project to the neurohypophysis and to the NTS, DMV and their vicinities, and that some of the caudally-projecting PVN neurons receive synaptic input from carotid baroreceptor reflex pathway, suggesting the possible involvement of these PVN neurons in central cardiovascular regulation.