Literature DB >> 6314892

Nucleic acid hybridization for measurement of effects of antiviral compounds on human cytomegalovirus DNA replication.

H Gadler.   

Abstract

A nucleic acid hybridization technique has been developed to study the effect of different antiviral compounds on the replication of human cytomegalovirus in vitro. One laboratory strain of human cytomegalovirus, Ad. 169, and six clinical isolates were studied. Doses needed for 50% inhibition of viral DNA replication were calculated for foscarnet, acyclovir, and arabinosyladenine. The mean 50% inhibition dose values obtained were 179 microM for foscarnet, 82 microM for acyclovir, and 44 microM for arabinosyladenine. This method yields values that agree with earlier reports, and it offers great advantages over usual methods to date for studying inhibition of viral DNA replication.

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Year:  1983        PMID： 6314892      PMCID： PMC185327          DOI： 10.1128/AAC.24.3.370

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  8 in total

1.  Characteristics of herpes simplex virus resistance to disodium phosphonoacetate.

Authors:  R G Duff; E E Robishaw; J C Mao; L R Overby
Journal:  Intervirology       Date:  1978       Impact factor: 1.763

2.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

3.  Comparison of the effects of arabinosyladenine, arabinosylhypoxanthine, and arabinosyladenine 5'-monophosphate against herpes simplex virus, varicella-zoster virus, and cytomegalovirus with their effects on cellular deoxyribonucleic acid synthesis.

Authors:  J F Gephart; A M Lerner
Journal:  Antimicrob Agents Chemother       Date:  1981-01       Impact factor: 5.191

4.  In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain.

Authors:  T R Chen
Journal:  Exp Cell Res       Date:  1977-02       Impact factor: 3.905

5.  Nucleic acid spot hybridization: rapid quantitative screening of lymphoid cell lines for Epstein-Barr viral DNA.

Authors:  J Brandsma; G Miller
Journal:  Proc Natl Acad Sci U S A       Date:  1980-11       Impact factor: 11.205

6.  Association between virus and cell DNA during latent cytomegalovirus infection in vitro.

Authors:  H Gadler; B Wahren
Journal:  Arch Virol       Date:  1983       Impact factor: 2.574

7.  Thymidine kinase not required for antiviral activity of acyclovir against mouse cytomegalovirus.

Authors:  W H Burns; J R Wingard; W J Bender; R Saral
Journal:  J Virol       Date:  1981-09       Impact factor: 5.103

8.  Influence of cells and virus multiplicity on the inhibition of herpesviruses with acycloguanosine.

Authors:  J Harmenberg; B Wahren; B Oberg
Journal:  Intervirology       Date:  1980       Impact factor: 1.763
  8 in total
  16 in total

Review 1.  Herpes simplex virus ocular infections: current concepts of acute, latent and reactivated disease.

Authors:  D Pavan-Langston
Journal:  Trans Am Ophthalmol Soc       Date:  1990

2.  Pyrrolo[2,3-d]pyrimidine nucleosides as inhibitors of human cytomegalovirus.

Authors:  S R Turk; C Shipman; R Nassiri; G Genzlinger; S H Krawczyk; L B Townsend; J C Drach
Journal:  Antimicrob Agents Chemother       Date:  1987-04       Impact factor: 5.191

3.  Activity of the cytomegalovirus genome in the presence of PPi analogs.

Authors:  B Wahren; U Rudén; H Gadler; B Oberg; B Eriksson
Journal:  J Virol       Date:  1985-12       Impact factor: 5.103

4.  Ganciclovir-resistant cytomegalovirus clinical isolates: mode of resistance to ganciclovir.

Authors:  S C Stanat; J E Reardon; A Erice; M C Jordan; W L Drew; K K Biron
Journal:  Antimicrob Agents Chemother       Date:  1991-11       Impact factor: 5.191

5.  Determination of inhibitory concentrations of antiviral agents in cell culture by use of an enzyme immunoassay with virus-specific, peroxidase-labeled monoclonal antibodies.

Authors:  F H van Tiel; W A Boere; T Harmsen; C A Kraaijeveld; H Snippe
Journal:  Antimicrob Agents Chemother       Date:  1985-05       Impact factor: 5.191

6.  1592U89, a novel carbocyclic nucleoside analog with potent, selective anti-human immunodeficiency virus activity.

Authors:  S M Daluge; S S Good; M B Faletto; W H Miller; M H St Clair; L R Boone; M Tisdale; N R Parry; J E Reardon; R E Dornsife; D R Averett; T A Krenitsky
Journal:  Antimicrob Agents Chemother       Date:  1997-05       Impact factor: 5.191

7.  Acyclic guanosine analogs as inhibitors of human cytomegalovirus.

Authors:  B Wahren; A Larsson; U Rudén; A Sundqvist; E Sølver
Journal:  Antimicrob Agents Chemother       Date:  1987-02       Impact factor: 5.191

8.  A human cytomegalovirus mutant resistant to the nucleoside analog 9-([2-hydroxy-1-(hydroxymethyl)ethoxy]methyl)guanine (BW B759U) induces reduced levels of BW B759U triphosphate.

Authors:  K K Biron; J A Fyfe; S C Stanat; L K Leslie; J B Sorrell; C U Lambe; D M Coen
Journal:  Proc Natl Acad Sci U S A       Date:  1986-11       Impact factor: 11.205

9.  Amplified DNAs in laboratory stocks of Leishmania tarentolae: extrachromosomal circles structurally and functionally similar to the inverted-H-region amplification of methotrexate-resistant Leishmania major.

Authors:  M L Petrillo-Peixoto; S M Beverley
Journal:  Mol Cell Biol       Date:  1988-12       Impact factor: 4.272

10.  Herpes simplex virus type 1 DNA cleavage and encapsidation require the product of the UL28 gene: isolation and characterization of two UL28 deletion mutants.

Authors:  L A Tengelsen; N E Pederson; P R Shaver; M W Wathen; F L Homa
Journal:  J Virol       Date:  1993-06       Impact factor: 5.103
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