| Literature DB >> 6314095 |
L Meile, A Kiener, T Leisinger.
Abstract
The archaebacterium Methanobacterium thermoautotrophicum Marburg (DSM 2133) was found to contain a plasmid (pME2001) in covalently closed circular form. It was isolated by CsCl gradient centrifugation of total DNA in the presence of ethidium bromide. Multimers up to the hexamer were observed upon agarose gel electrophoresis and electron microscopy of a purified plasmid preparation. A restriction map was constructed. The length of plasmid pME2001 was determined to be approximately 4,500 bp. Southern hybridization of plasmid DNA to DNA extracted from Methanobacterium thermoautotrophicum delta H (DSM1053) revealed the presence of a plasmid with homologous sequences in the delta H strain.Entities:
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Year: 1983 PMID: 6314095 DOI: 10.1007/bf00425766
Source DB: PubMed Journal: Mol Gen Genet ISSN: 0026-8925