| Literature DB >> 6313726 |
K Bellamy, P S Gardner, M H Hambling, S Rice, A F Bradburne.
Abstract
A simple method for the detection of human rotavirus in stools is described, using a double antibody sandwich enzyme-linked immunosorbent assay. Polysterene microtitre plates were used as solid phase. Four capture antibodies were tried, bovine, egg-derived, guinea pig and monoclonal antibody to rotavirus. Both bovine and egg-derived antirotavirus labelled with horseradish peroxidase were used as the detecting antibodies. The results obtained were compared with a commercially available ELISA, Rotazyme (Abbott Laboratories), and also with the direct detection of rotavirus by electron microscopy. Bovine antibody was found to be an unsuitable capture antibody due to non-specific false positive reactions.Entities:
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Year: 1983 PMID： 6313726 DOI： 10.1016/0166-0934(83)90093-9
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014