Literature DB >> 6313475

Molecular cloning in Bacillus subtilis of a Bacillus licheniformis gene encoding a thermostable alpha amylase.

S A Ortlepp, J F Ollington, D J McConnell.   

Abstract

A resident-plasmid cloning system developed for Bacillus subtilis has been used to isolate recombinant plasmids carrying DNA from Bacillus licheniformis which confer alpha-amylase activity on alpha-amylase-negative mutants of B. subtilis. These plasmids contain a 3550-bp insert at the EcoRI site of the plasmid pBD64. Subcloning various lengths of the B. licheniformis DNA has localised the gene to a 2550-bp BclI fragment. We present evidence that the cloned fragment codes for a B. licheniformis heat-stable alpha-amylase with a temperature optimum of 93 degrees C. The foreign gene is expressed efficiently in B. subtilis and is stably maintained.

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Year:  1983        PMID： 6313475     DOI： 10.1016/0378-1119(83)90017-3

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  14 in total

1.  Characterization of PBSX, a defective prophage of Bacillus subtilis.

Authors:  H E Wood; M T Dawson; K M Devine; D J McConnell
Journal:  J Bacteriol       Date:  1990-05       Impact factor: 3.490

2.  Molecular cloning, structure, promoters and regulatory elements for transcription of the Bacillus megaterium encoded regulon for xylose utilization.

Authors:  T Rygus; A Scheler; R Allmansberger; W Hillen
Journal:  Arch Microbiol       Date:  1991       Impact factor: 2.552

3.  Integrable alpha-amylase plasmid for generating random transcriptional fusions in Bacillus subtilis.

Authors:  C O'Kane; M A Stephens; D McConnell
Journal:  J Bacteriol       Date:  1986-11       Impact factor: 3.490

4.  Genetic engineering of microorganisms for biotechnology.

Authors:  E J Stellwag; J E Brenchley
Journal:  Microb Ecol       Date:  1986-03       Impact factor: 4.552

5.  Chaperone-mediated activation in vivo of a Pseudomonas cepacia lipase.

Authors:  J L Aamand; A H Hobson; C M Buckley; S T Jørgensen; B Diderichsen; D J McConnell
Journal:  Mol Gen Genet       Date:  1994-12-01

6.  Expression of alpha-amylase in Bacillus licheniformis.

Authors:  D M Rothstein; P E Devlin; R L Cate
Journal:  J Bacteriol       Date:  1986-11       Impact factor: 3.490

7.  The influence of secretory-protein charge on late stages of secretion from the Gram-positive bacterium Bacillus subtilis.

Authors:  K Stephenson; C L Jensen; S T Jørgensen; J H Lakey; C R Harwood
Journal:  Biochem J       Date:  2000-08-15       Impact factor: 3.857

8.  Overproduction, isolation, and DNA-binding characteristics of Xre, the repressor protein from the Bacillus subtilis defective prophage PBSX.

Authors:  G E McDonnell; D J McConnell
Journal:  J Bacteriol       Date:  1994-09       Impact factor: 3.490

9.  Plasmid instability in an industrial strain of Bacillus subtilis grown in chemostat culture.

Authors:  G T Fleming; J W Patching
Journal:  J Ind Microbiol       Date:  1994-03

10.  Nucleotide sequence of the 5' region of the Bacillus licheniformis alpha-amylase gene: comparison with the B. amyloliquefaciens gene.

Authors:  M A Stephens; S A Ortlepp; J F Ollington; D J McConnell
Journal:  J Bacteriol       Date:  1984-04       Impact factor: 3.490
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