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Literature DB >> 6313345

Poly(ADP-ribose) polymerase auto-modification and interaction with DNA: electron microscopic visualization.

G de Murcia, J Jongstra-Bilen, M E Ittel, P Mandel, E Delain.

Abstract

The interaction between purified calf thymus poly(ADP-ribose) polymerase and its activating co-purified DNA (sDNA) was investigated by electron microscopy. We have shown that the enzyme-DNA complex possesses a nucleosome-like structure. The enzyme-bound DNA (sDNA) was found to be enriched in single-stranded regions and branched structures, presumed to be replication forks. The auto-ribosylated polymerase as well as the branched poly(ADP-ribose) formed were visualized by dark field electron microscopy during the auto-ADP-ribosylation reaction and the possible mechanism of this phenomenon is discussed.

Entities: Chemical

Mesh：

Substances：

Year: 1983 PMID： 6313345 PMCID： PMC555058 DOI： 10.1002/j.1460-2075.1983.tb01460.x

Source DB: PubMed Journal: EMBO J ISSN： 0261-4189 Impact factor: 11.598

35 in total

1. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.

Authors: T Maniatis; A Jeffrey; H van deSande
Journal: Biochemistry Date: 1975-08-26 Impact factor: 3.162

2. Stimulation of poly(ADP-ribose) polymerase activity by the anti-tumour antibiotic, streptozotocin.

Authors: W J Whish; M I Davies; S Shall
Journal: Biochem Biophys Res Commun Date: 1975-07-22 Impact factor: 3.575

3. Release of template restriction for DNA synthesis by poly ADP(ribose) polymerase during the HeLa cell cycle.

Authors: M Smulson; P Stark; M Gazzoli; J Roberts
Journal: Exp Cell Res Date: 1975-01 Impact factor: 3.905

4. Purification and properties of calf thymus polyadenosine diphosphate ribose polymerase.

Authors: H Okazaki; C Niedergang; P Mandel
Journal: FEBS Lett Date: 1976-03-01 Impact factor: 4.124

5. Interrelationship between poly (ADP-Rib) synthesis, intracellular NAD levels, and muscle or cartilage differentiation from mesodermal cells of embryonic chick limb.

Authors: A I Caplan; M J Rosenberg
Journal: Proc Natl Acad Sci U S A Date: 1975-05 Impact factor: 11.205

Review 6. Poly(ADP-ribose) and ADP-ribosylation of proteins.

Authors: H Hilz; P Stone
Journal: Rev Physiol Biochem Pharmacol Date: 1976 Impact factor: 5.545

7. Purification and properties of calf thymus poly adenosine diphosphate ribose polymerase.

Authors: P Mandel; H Okazaki; C Niedergang
Journal: FEBS Lett Date: 1977-12-15 Impact factor: 4.124

8. A functional role of polyADPR in DNA synthesis.

Authors: L Burzio; S S Koide
Journal: Biochem Biophys Res Commun Date: 1970-09-10 Impact factor: 3.575

9. A new preparation method for dark-field electron microscopy of biomacromolecules.

Authors: J Dubochet; M Ducommun; M Zollinger; E Kellenberger
Journal: J Ultrastruct Res Date: 1971-04

10. Superstructure and CD spectrum as probes of chromatin integrity.

Authors: G de Murcia; G C Das; M Erard; M Daune
Journal: Nucleic Acids Res Date: 1978-02 Impact factor: 16.971

23 in total

1. Visualization of DNA and RNA molecules, and protein-DNA complexes for electron microscopy.

Authors: M A Hajibagheri
Journal: Mol Biotechnol Date: 2000-06 Impact factor: 2.695

2. Enzymatic synthesis and structural characterization of 13C, 15N-poly(ADP-ribose).

Authors: Heather L Schultheisz; Blair R Szymczyna; James R Williamson
Journal: J Am Chem Soc Date: 2009-10-14 Impact factor: 15.419

Review 3. Poly(ADP-ribosyl)ation reactions in the regulation of nuclear functions.

Authors: D D'Amours; S Desnoyers; I D'Silva; G G Poirier
Journal: Biochem J Date: 1999-09-01 Impact factor: 3.857

4. ADP-ribosyltransferase in isolated nuclei during the cell cycle of Physarum polycephalum.

Authors: P Gröbner; P Loidl
Journal: Biochem J Date: 1985-11-15 Impact factor: 3.857

5. A dominant-negative mutant of human poly(ADP-ribose) polymerase affects cell recovery, apoptosis, and sister chromatid exchange following DNA damage.

Authors: V Schreiber; D Hunting; C Trucco; B Gowans; D Grunwald; G De Murcia; J M De Murcia
Journal: Proc Natl Acad Sci U S A Date: 1995-05-23 Impact factor: 11.205