Literature DB >> 6312971

1H-n.m.r. investigation of the interaction between cytochrome c and cytochrome b5.

C G Eley, G R Moore.   

Abstract

The interaction between eukaryotic cytochrome c and the tryptic fragment of bovine liver microsomal cytochrome b5 was studied by 1H-n.m.r. spectroscopy, and a procedure was developed that may be generally applicable to the study of macromolecular interactions by n.m.r. At pH6.3 (27 degrees C, I approx. 0.04) the two ferricytochromes were found to form a 1:1 complex with an association constant of approx. 10(3) M -1. The protein--protein-interaction region was found to encompass the region of the surface of horse cytochrome c that includes Ile-81, Phe-82, Ala-83 and Ile-85, and Lys-13 and Lys-72 of horse cytochrome c were suggested to be involved in two important intermolecular interactions. Me3Lys-72 of Candida krusei cytochrome c was shown to be involved in the interaction.

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Year:  1983        PMID： 6312971      PMCID： PMC1152358          DOI： 10.1042/bj2150011

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  43 in total

1.  An hypothetical structure for an intermolecular electron transfer complex of cytochromes c and b5.

Authors:  F R Salemme
Journal:  J Mol Biol       Date:  1976-04-15       Impact factor: 5.469

2.  Effect of modification of individual cytochrome c lysines on the reaction with cytochrome b5.

Authors:  S Ng; M B Smith; H T Smith; F Millett
Journal:  Biochemistry       Date:  1977-11-15       Impact factor: 3.162

3.  Assignment of the heme c resonances in the 360 MHz H NMR spectra of cytochrome c.

Authors:  R M Keller; K Wüthrich
Journal:  Biochim Biophys Acta       Date:  1978-03-28

4.  Ion binding to cytochrome c studied by nuclear magnetic quadrupole relaxation.

Authors:  T Andersson; E Thulin; S Forsén
Journal:  Biochemistry       Date:  1979-06-12       Impact factor: 3.162

5.  Contact-shifted resonances in the 1H NMR spectra of cytochrome b5. Resonance identification and spin density distribution in the heme group.

Authors:  R Keller; O Groudinsky; K Wüthrich
Journal:  Biochim Biophys Acta       Date:  1976-04-14

6.  A new method for simultaneous purification of cytochrome b5 and NADPH-cytochrome c reductase from rat liver microsomes.

Authors:  T Omura; S Takesue
Journal:  J Biochem       Date:  1970-02       Impact factor: 3.387

7.  Electrostatic interactions in cytochrome c. The role of interactions between residues 13 and 90 and residues 79 and 47 in stabilizing the heme crevice structure.

Authors:  N Osheroff; D Borden; W H Koppenol; E Margoliash
Journal:  J Biol Chem       Date:  1980-02-25       Impact factor: 5.157

8.  Complete amino acid sequence of the heme-binding core in bakers' yeast cytochrome b2 (L-(+)-lactate dehydrogenase).

Authors:  B Guiard; F Lederer
Journal:  Biochimie       Date:  1976       Impact factor: 4.079

9.  Redox properties of microsomal reduced nicotinamide adenine dinucleotide-cytochrome b5 reductase and cytochrome b5.

Authors:  T Iyanagi
Journal:  Biochemistry       Date:  1977-06-14       Impact factor: 3.162

10.  Reduction of respiratory-chain cytochrome b by lactate in Saccharomyces cerevisiae.

Authors:  M Briquet; B Purnelle; D S Beattie; A Goffeau
Journal:  Eur J Biochem       Date:  1982-10
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  10 in total

1.  Electrostatic and steric control of electron self-exchange in cytochromes c, c551, and b5.

Authors:  D W Dixon; X Hong; S E Woehler
Journal:  Biophys J       Date:  1989-08       Impact factor: 4.033

2.  A further investigation of the cytochrome b5-cytochrome c complex.

Authors:  Lucia Banci; Ivano Bertini; Isabella C Felli; Ludwig Krippahl; Karel Kubicek; José J G Moura; Antonio Rosato
Journal:  J Biol Inorg Chem       Date:  2003-07-19       Impact factor: 3.358

3.  Side chain mobility as monitored by CH-CH cross correlation: the example of cytochrome b5.

Authors:  L Banci; I Bertini; I C Felli; P Hajieva; M S Viezzoli
Journal:  J Biomol NMR       Date:  2001-05       Impact factor: 2.835

Review 4.  Electron transfer from cytochrome b5 to cytochrome c.

Authors:  B Durham; J L Fairris; M McLean; F Millett; J R Scott; S G Sligar; A Willie
Journal:  J Bioenerg Biomembr       Date:  1995-06       Impact factor: 2.945

Review 5.  Experimental and theoretical analysis of the interaction between cytochrome c and cytochrome b5.

Authors:  A G Mauk; M R Mauk; G R Moore; S H Northrup
Journal:  J Bioenerg Biomembr       Date:  1995-06       Impact factor: 2.945

6.  Effects of charged amino-acid mutation on the solution structure of cytochrome b(5) and binding between cytochrome b(5) and cytochrome c.

Authors:  C Qian; Y Yao; K Ye; J Wang; W Tang; Y Wang; W Wang; J Lu; Y Xie; Z Huang
Journal:  Protein Sci       Date:  2001-12       Impact factor: 6.725

7.  N epsilon,N epsilon-dimethyl-lysine cytochrome c as an NMR probe for lysine involvement in protein-protein complex formation.

Authors:  G R Moore; M C Cox; D Crowe; M J Osborne; F I Rosell; J Bujons; P D Barker; M R Mauk; A G Mauk
Journal:  Biochem J       Date:  1998-06-01       Impact factor: 3.857

8.  The orientations of cytochrome c in the highly dynamic complex with cytochrome b5 visualized by NMR and docking using HADDOCK.

Authors:  Alexander N Volkov; Davide Ferrari; Jonathan A R Worrall; Alexandre M J J Bonvin; Marcellus Ubbink
Journal:  Protein Sci       Date:  2005-02-02       Impact factor: 6.725

9.  Characterization and calculation of a cytochrome c-cytochrome b5 complex using NMR data.

Authors:  Shashank Deep; Sang-Choul Im; Erik R P Zuiderweg; Lucy Waskell
Journal:  Biochemistry       Date:  2005-08-09       Impact factor: 3.162

10.  Kinetic and Structural Characterization of the Effects of Membrane on the Complex of Cytochrome b 5 and Cytochrome c.

Authors:  Katherine A Gentry; Elke Prade; Carlo Barnaba; Meng Zhang; Mukesh Mahajan; Sang-Choul Im; G M Anantharamaiah; Satoshi Nagao; Lucy Waskell; Ayyalusamy Ramamoorthy
Journal:  Sci Rep       Date:  2017-08-10       Impact factor: 4.379
  10 in total

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