Literature DB >> 631288

Large-scale purification of hepatitis B surface antigen using affinity chromatography.

A R Neurath, A M Prince, J Giacalone.   

Abstract

Large-scale purification of hepatitis B surface antigen, applicable to the preparation of potential vaccines for prevention of hepatitis B, is described. The method involves the following steps: precipitation of serum with polyethylene glycol 6000, affinity chromatography on concanavalin A-Sepharose and on omega-aminononyl-Sepharose, and isopycnic centrifugation.

Entities:  

Mesh：

Substances：

Year:  1978        PMID： 631288     DOI： 10.1007/bf01923068

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  3 in total

1.  Affinity chromatography of hepatitis B antigen on concanavalin A linked to sepharose.

Authors:  A R Neurath; A M Prince; A Lippin
Journal:  J Gen Virol       Date:  1973-06       Impact factor: 3.891

2.  Hepatitis B antigens: separation of two populations differing in particle size distribution.

Authors:  A R Neurath; L Cosio; A M Prince; A Lippin; H Ikram
Journal:  Proc Soc Exp Biol Med       Date:  1973-06

3.  Hydrophobic chromatography of hepatitis B surface antigen on 1,9-diaminononane or 1,10-diaminodecane linked to agarose.

Authors:  A R Neurath; S Lerman; M Chen; A M Prince
Journal:  J Gen Virol       Date:  1975-08       Impact factor: 3.891
  3 in total
  2 in total

1.  Specificity of antibodies elicited by a synthetic peptide having a sequence in common with a fragment of a virus protein, the hepatitis B surface antigen.

Authors:  A R Neurath; S B Kent; N Strick
Journal:  Proc Natl Acad Sci U S A       Date:  1982-12       Impact factor: 11.205

2.  Radioimmunoassays of hidden viral antigens.

Authors:  A R Neurath; N Strick; L Baker; S Krugman
Journal:  Proc Natl Acad Sci U S A       Date:  1982-07       Impact factor: 11.205
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.