Literature DB >> 6312843

Simultaneous staining of proteins during polyacrylamide gel electrophoresis in acidic gels by countermigration of Coomassie brilliant blue R-250.

G Varghese, A M Diwan.   

Abstract

A method for the simultaneous staining of proteins during polyacrylamide gel electrophoresis with Coomassie brilliant blue R-250 at pH 2.5 is described. Calf thymus whole histone and cytochrome c were stained by this method and the results obtained were similar to that obtained by staining after electrophoresis.

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Year:  1983        PMID: 6312843     DOI: 10.1016/0003-2697(83)90037-4

Source DB:  PubMed          Journal:  Anal Biochem        ISSN: 0003-2697            Impact factor:   3.365


  11 in total

1.  Cloning of exo-β-1,3-glucanase gene from a marine yeast Williopsis saturnus and its overexpression in Yarrowia lipolytica.

Authors:  Ying Peng; Guang-Lei Liu; Xin-Jun Yu; Xiang-Hong Wang; Li Jing; Zhen-Ming Chi
Journal:  Mar Biotechnol (NY)       Date:  2010-03-25       Impact factor: 3.619

2.  Melanin biosynthesis in the desert-derived Aureobasidium melanogenum XJ5-1 is controlled mainly by the CWI signal pathway via a transcriptional activator Cmr1.

Authors:  Hong Jiang; Zhe Chi; Guang-Lei Liu; Zhong Hu; Shuang-Zhi Zhao; Zhen-Ming Chi
Journal:  Curr Genet       Date:  2019-07-01       Impact factor: 3.886

3.  Production, Purification, and Gene Cloning of a β-Fructofuranosidase with a High Inulin-hydrolyzing Activity Produced by a Novel Yeast Aureobasidium sp. P6 Isolated from a Mangrove Ecosystem.

Authors:  Hong Jiang; Yan Ma; Zhe Chi; Guang-Lei Liu; Zhen-Ming Chi
Journal:  Mar Biotechnol (NY)       Date:  2016-06-28       Impact factor: 3.619

4.  Production, Gene Cloning, and Overexpression of a Laccase in the Marine-Derived Yeast Aureobasidium melanogenum Strain 11-1 and Characterization of the Recombinant Laccase.

Authors:  Thu Aung; Hong Jiang; Cheng-Cheng Chen; Guang-Lei Liu; Zhong Hu; Zhen-Ming Chi; Zhe Chi
Journal:  Mar Biotechnol (NY)       Date:  2018-11-19       Impact factor: 3.619

5.  beta-1,3-glucanase inhibits activity of the killer toxin produced by the marine-derived yeast Williopsis saturnus WC91-2.

Authors:  Ying Peng; Zhenming Chi; Xianghong Wang; Jing Li
Journal:  Mar Biotechnol (NY)       Date:  2009-11-26       Impact factor: 3.619

6.  Purification and characterization of killer toxin from a marine yeast Pichia anomala YF07b against the pathogenic yeast in crab.

Authors:  X Wang; Z Chi; L Yue; J Li
Journal:  Curr Microbiol       Date:  2007-08-09       Impact factor: 2.188

7.  Purification and characterization of extracellular phytase from a marine yeast Kodamaea ohmeri BG3.

Authors:  Xiaoyu Li; Zhenming Chi; Zhiqiang Liu; Jing Li; Xianghong Wang; Nalini Yasoda Hirimuthugoda
Journal:  Mar Biotechnol (NY)       Date:  2007-11-27       Impact factor: 3.619

8.  Purification and characterization of an alkaline protease from the marine yeast Aureobasidium pullulans for bioactive peptide production from different sources.

Authors:  Chunling Ma; Xiumei Ni; Zhenming Chi; Liyan Ma; Lingmei Gao
Journal:  Mar Biotechnol (NY)       Date:  2007-03-07       Impact factor: 3.619

9.  Purification and characterization of a novel chlorpyrifos hydrolase from Cladosporium cladosporioides Hu-01.

Authors:  Yan Gao; Shaohua Chen; Meiying Hu; Qiongbo Hu; Jianjun Luo; Yanan Li
Journal:  PLoS One       Date:  2012-06-05       Impact factor: 3.240

10.  New Lipase for Biodiesel Production: Partial Purification and Characterization of LipSB 25-4.

Authors:  Aysel Ugur; Nurdan Sarac; Rukiye Boran; Berk Ayaz; Ozgur Ceylan; Gulten Okmen
Journal:  ISRN Biochem       Date:  2014-03-10
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