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Literature DB >> 6312836

A novel chromatographic procedure for purification of bacterial plasmids.

Abstract

A new, rapid procedure for purifying bacterial plasmids with high recovery is described. The sequence of operations consists essentially of treatment with alkali, ribonuclease, and proteinase K, followed by chisam extraction and gel filtration on Sephacryl S-1000, and finally a precipitation step using isopropanol at room temperature. The method gives rather good yields of plasmid DNA of high purity, and lends itself to scaling up.

Entities: Chemical

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Year: 1983 PMID： 6312836 DOI： 10.1016/0003-2697(83)90451-7

Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365

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Cited

8 in total

1. Efficient in vitro import of a cytosolic heat shock protein into pea chloroplasts.

Authors: T H Lubben; K Keegstra
Journal: Proc Natl Acad Sci U S A Date: 1986-08 Impact factor: 11.205

2. Cloning and characterization of a cDNA encoding the chloroplastic copper/zinc-superoxide dismutase from pea.

Authors: J R Scioli; B A Zilinskas
Journal: Proc Natl Acad Sci U S A Date: 1988-10 Impact factor: 11.205

3. Expression in Escherichia coli K-12 of the 76,000-dalton iron-regulated outer membrane protein of Shigella flexneri confers sensitivity to cloacin DF13 in the absence of Shigella O antigen.

Authors: P Derbyshire; T Baldwin; P Stevenson; E Griffiths; M Roberts; P Williams; T L Hale; S B Formal
Journal: Infect Immun Date: 1989-09 Impact factor: 3.441

A novel chromatographic procedure for purification of bacterial plasmids.

1. Efficient in vitro import of a cytosolic heat shock protein into pea chloroplasts.

2. Cloning and characterization of a cDNA encoding the chloroplastic copper/zinc-superoxide dismutase from pea.

3. Expression in Escherichia coli K-12 of the 76,000-dalton iron-regulated outer membrane protein of Shigella flexneri confers sensitivity to cloacin DF13 in the absence of Shigella O antigen.

4. Primary structure of a mouse mastocytoma proteoglycan core protein.

5. Endotoxin and tumor necrosis factor induce interleukin-1 gene expression in adult human vascular endothelial cells.

6. Mapping of a cloned glucosyltransferase gene in Streptococcus mutans.

7. Inducible interleukin-1 gene expression in human vascular smooth muscle cells.

8. Human interleukin 1 induces interleukin 1 gene expression in human vascular smooth muscle cells.