Literature DB >> 6312382

Freezing eliminates a specific population of L-glutamate receptors in synaptic membranes.

G E Fagg, E E Mena, D T Monaghan, C W Cotman.   

Abstract

The binding of L-[3H]glutamate (L-Glu) to freeze-thawed synaptic membranes (SPMs) exhibited saturation kinetics, with Kd 507 nM and Bmax 6.99 pmol/mg protein. The effects of ions, the susceptibility to Triton X-100 and the pharmacological properties of the binding indicated that those sites detected in freeze-thawed SPMs were only of the Cl-/Ca2+-independent type. The Cl-/Ca2+-dependent (2-amino-4-phosphonobutyrate-sensitive) L-Glu binding sites which are additionally present in fresh SPMs are abolished by freezing.

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Year:  1983        PMID： 6312382     DOI： 10.1016/0304-3940(83)90033-2

Source DB:  PubMed          Journal:  Neurosci Lett        ISSN： 0304-3940            Impact factor:   3.046


  7 in total

1.  Age-associated hypothalamic glutamate receptor density: effect of dietary protein.

Authors:  L Chakrabarti; B C Bandyopadhyay; M K Poddar
Journal:  Neurochem Res       Date:  2001-02       Impact factor: 3.996

2.  Saving the "library of life".

Authors:  G Benford
Journal:  Proc Natl Acad Sci U S A       Date:  1992-11-15       Impact factor: 11.205

3.  Evidence for heterogenous glycine domains but conserved multiple states of the excitatory amino acid recognition site of the NMDA receptor: regional binding studies with [3H]glycine and [3H]L-glutamate.

Authors:  R D O'Shea; D T Manallack; E L Conway; L D Mercer; P M Beart
Journal:  Exp Brain Res       Date:  1991       Impact factor: 1.972

4.  Analysis of glutamate receptors in primary cultured neurons from fetal rat forebrain.

Authors:  J Oillet; F Nicolas; V Koziel; J L Daval
Journal:  Neurochem Res       Date:  1995-06       Impact factor: 3.996

5.  Selective association of N-methyl aspartate and quisqualate types of L-glutamate receptor with brain postsynaptic densities.

Authors:  G E Fagg; A Matus
Journal:  Proc Natl Acad Sci U S A       Date:  1984-11       Impact factor: 11.205

6.  Cl-/Ca2+-dependent L-glutamate binding sites do not correspond to 2-amino-4-phosphonobutanoate-sensitive excitatory amino acid receptors.

Authors:  G E Fagg; T H Lanthorn
Journal:  Br J Pharmacol       Date:  1985-11       Impact factor: 8.739

7.  Differentiation of Cl-/Ca2+-dependent and sodium dependent 3H-glutamate binding to cortical membranes from rat brain by high energy radiation inactivation analysis.

Authors:  T Honoré; J Drejer; M Nielsen; C Braestrup
Journal:  J Neural Transm       Date:  1986       Impact factor: 3.575
  7 in total

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