| Literature DB >> 6311809 |
Abstract
Plasmid pIG7 DNA cloned in Pseudomonas putida with the broad-host-range vectors pRK290 and pKT240 expresses the genes encoding nephthalene oxidation in the presence of the intermediate substrate, salicylate, or the gratuitous inducer, anthranilate. Two operons, nahAF and nahGK, cloned from the EcoRI fragment A (25 kilobases) are under wild-type regulation by the nahR locus. Deletion plasmids provide a restriction map of both operons. Double transformants containing structural and regulatory cistron nahR in trans are used to demonstrate positive control of expression.Entities:
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Year: 1983 PMID: 6311809 PMCID: PMC215054 DOI: 10.1128/jb.156.1.89-94.1983
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490