Literature DB >> 6311805

Comparison of two methods of genetic exchange in determination of the genetic locus of the structural gene for Pseudomonas aeruginosa elastase.

T R Howe, B Wretlind, B H Iglewski.   

Abstract

A mutation, lasA1, that results in the synthesis of enzymatically inactive elastase protein was mapped by R68.45 plasmid-mediated conjugation and transposon-facilitated recombination to ca. 75 min on the Pseudomonas aeruginosa PAO chromosome. Since immunologically cross-reactive material is produced, the mutation presumably arose within the elastase structural gene.

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Year:  1983        PMID: 6311805      PMCID: PMC215050          DOI: 10.1128/jb.156.1.58-61.1983

Source DB:  PubMed          Journal:  J Bacteriol        ISSN: 0021-9193            Impact factor:   3.490


  15 in total

1.  PSEUDOMONAS AERUGINOSA ELASTASE. ISOLATION, CRYSTALLIZATION, AND PRELIMINARY CHARACTERIZATION.

Authors:  K MORIHARA; H TSUZUKI; T OKA; H INOUE; M EBATA
Journal:  J Biol Chem       Date:  1965-08       Impact factor: 5.157

2.  Acetylornithinase of Escherichia coli: partial purification and some properties.

Authors:  H J VOGEL; D M BONNER
Journal:  J Biol Chem       Date:  1956-01       Impact factor: 5.157

3.  Chromosome mapping in Pseudomonas aeruginosa PAT.

Authors:  J M Watson; B W Holloway
Journal:  J Bacteriol       Date:  1978-03       Impact factor: 3.490

4.  Genetic circularity of the Pseudomonas aeruginosa PAO chromosome.

Authors:  P L Royle; H Matsumoto; B W Holloway
Journal:  J Bacteriol       Date:  1981-01       Impact factor: 3.490

Review 5.  Comparative specificity of microbial proteinases.

Authors:  K Morihara
Journal:  Adv Enzymol Relat Areas Mol Biol       Date:  1974

6.  Effects of protease and elastase from Pseudomonas aeruginosa on skin.

Authors:  K Kawaharajo; J Y Homma; Y Aoyama; K Okada; K Morihara
Journal:  Jpn J Exp Med       Date:  1975-04

7.  Chromosome mobilization by the R plasmid R68.45: a tool in Pseudomonas genetics.

Authors:  D Haas; B W Holloway
Journal:  Mol Gen Genet       Date:  1978-01-17

8.  Isolation and characterization of Pseudomonas aeruginosa PAO mutant that produces altered elastase.

Authors:  D E Ohman; S J Cryz; B H Iglewski
Journal:  J Bacteriol       Date:  1980-06       Impact factor: 3.490

9.  In Vivo Studies with the Partially Purified Protease (Elastase) from Pseudomonas aeruginosa.

Authors:  G Meinke; J Barum; B Rosenberg; R Berk
Journal:  Infect Immun       Date:  1970-11       Impact factor: 3.441

10.  Isolation of an Hfr donor of Pseudomonas aeruginosa PAO by insertion of the plasmid RP1 into the tryptophan synthase gene.

Authors:  D Haas; J Watson; R Krieg; T Leisinger
Journal:  Mol Gen Genet       Date:  1981
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  7 in total

1.  Activation of an elastase precursor by the lasA gene product of Pseudomonas aeruginosa.

Authors:  J B Goldberg; D E Ohman
Journal:  J Bacteriol       Date:  1987-10       Impact factor: 3.490

2.  Mapping of mutations in Pseudomonas aeruginosa defective in pyoverdin production.

Authors:  R Ankenbauer; L F Hanne; C D Cox
Journal:  J Bacteriol       Date:  1986-07       Impact factor: 3.490

3.  Genetic mapping and characterization of Pseudomonas aeruginosa mutants defective in the formation of extracellular proteins.

Authors:  B Wretlind; O R Pavlovskis
Journal:  J Bacteriol       Date:  1984-06       Impact factor: 3.490

4.  Cloning and characterization of elastase genes from Pseudomonas aeruginosa.

Authors:  P A Schad; R A Bever; T I Nicas; F Leduc; L F Hanne; B H Iglewski
Journal:  J Bacteriol       Date:  1987-06       Impact factor: 3.490

5.  Cloning and transcriptional regulation of the elastase lasA gene in mucoid and nonmucoid Pseudomonas aeruginosa.

Authors:  J B Goldberg; D E Ohman
Journal:  J Bacteriol       Date:  1987-03       Impact factor: 3.490

6.  Nucleotide sequence and expression in Escherichia coli of the Pseudomonas aeruginosa lasA gene.

Authors:  P A Schad; B H Iglewski
Journal:  J Bacteriol       Date:  1988-06       Impact factor: 3.490

7.  lasA and lasB genes of Pseudomonas aeruginosa: analysis of transcription and gene product activity.

Authors:  D S Toder; S J Ferrell; J L Nezezon; L Rust; B H Iglewski
Journal:  Infect Immun       Date:  1994-04       Impact factor: 3.441

  7 in total

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