Literature DB >> 6311687

Molecular cloning and expression of a Bacillus subtilis beta-glucanase gene in Escherichia coli.

B A Cantwell, D J McConnell.   

Abstract

A Bacillus subtilis gene coding for an endo-beta-1,3-1,4-glucanase has been transferred to Escherichia coli by molecular cloning using bacteriophage lambda and plasmid vectors. The gene is contained within a 1.6-kb EcoRI-PvuI DNA fragment and directs the synthesis in E. coli of a beta-glucanase which specifically degrades barley glucan and lichenan. A novel dye-staining method has been developed to detect beta-glucanase activity in colonies on agar plates.

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Year:  1983        PMID： 6311687     DOI： 10.1016/0378-1119(83)90053-7

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  22 in total

1.  Potential of conjugal transfer as a strategy for the introduction of recombinant genetic material into strains of lactobacillus helveticus

Authors: 
Journal:  Appl Environ Microbiol       Date:  1999-05       Impact factor: 4.792

2.  Molecular cloning in Lactobacillus helveticus by plasmid pSA3::pVA797 co-integrate formation and conjugal transfer.

Authors:  K Thompson; M Collins
Journal:  Appl Microbiol Biotechnol       Date:  1991-06       Impact factor: 4.813

3.  Characterization of the sacQ genes from Bacillus licheniformis and Bacillus subtilis.

Authors:  A Amory; F Kunst; E Aubert; A Klier; G Rapoport
Journal:  J Bacteriol       Date:  1987-01       Impact factor: 3.490

4.  Expression of the cloned endo-1,3-1,4-β-glucanase gene of Bacillus subtilis in Saccharomyces cerevisiae.

Authors:  E Hinchliffe; W G Box
Journal:  Curr Genet       Date:  1984-08       Impact factor: 3.886

5.  Properties of a Clostridium thermocellum Endoglucanase Produced in Escherichia coli.

Authors:  W H Schwarz; F Gräbnitz; W L Staudenbauer
Journal:  Appl Environ Microbiol       Date:  1986-06       Impact factor: 4.792

6.  Molecular cloning of an endoglucanase gene from an alkalophilic Bacillus sp. and its expression in Escherichia coli.

Authors:  J M Kim; I S Kong; J H Yu
Journal:  Appl Environ Microbiol       Date:  1987-11       Impact factor: 4.792

7.  Expression of a cloned beta-glucanase gene from Bacillus amyloliquefaciens in an Escherichia coli relA strain after plasmid amplification.

Authors:  M Hecker; S Riethdorf; C Bauer; A Schroeter; R Borriss
Journal:  Mol Gen Genet       Date:  1988-12

8.  5'-noncoding region sacR is the target of all identified regulation affecting the levansucrase gene in Bacillus subtilis.

Authors:  S Aymerich; G Gonzy-Tréboul; M Steinmetz
Journal:  J Bacteriol       Date:  1986-06       Impact factor: 3.490

9.  The DNA sequence of the gene and genetic control sites for the excreted B. subtilis enzyme beta-glucanase.

Authors:  N Murphy; D J McConnell; B A Cantwell
Journal:  Nucleic Acids Res       Date:  1984-07-11       Impact factor: 16.971

10.  Two beta-glycanase genes are clustered in Bacillus polymyxa: molecular cloning, expression, and sequence analysis of genes encoding a xylanase and an endo-beta-(1,3)-(1,4)-glucanase.

Authors:  M J Gosalbes; J A Pérez-González; R González; A Navarro
Journal:  J Bacteriol       Date:  1991-12       Impact factor: 3.490
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